Construct: ORF ccsbBroad304_08013
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007751.1_s304c1, BRDN0000461014
- Derived from:
- ccsbBroadEn_08013
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANKRD2 (26287)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08013
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26287 | ANKRD2 | ankyrin repeat domain 2 | NM_020349.4 | 99.9% | 100% | 936A>G |
| 2 | human | 26287 | ANKRD2 | ankyrin repeat domain 2 | NM_001346793.2 | 92.4% | 92.5% | 0_1ins81;855A>G |
| 3 | human | 26287 | ANKRD2 | ankyrin repeat domain 2 | NM_001129981.3 | 90.7% | 90.8% | 734_735ins99;837A>G |
| 4 | human | 26287 | ANKRD2 | ankyrin repeat domain 2 | NM_001291219.3 | 83.2% | 83.3% | 0_1ins81;653_654ins99;756A>G |
| 5 | human | 26287 | ANKRD2 | ankyrin repeat domain 2 | NM_001291218.1 | 80.6% | 80.7% | 1_258del;1194A>G |
| 6 | mouse | 56642 | Ankrd2 | ankyrin repeat domain 2 (st... | NM_020033.1 | 78% | 81.3% | (many diffs) |
| 7 | mouse | 56642 | Ankrd2 | ankyrin repeat domain 2 (st... | XM_017318264.1 | 71.3% | 73.8% | (many diffs) |
| 8 | mouse | 56642 | Ankrd2 | ankyrin repeat domain 2 (st... | XM_006527234.3 | 69.8% | 73.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1149
- ORF length:
- 1080
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaaaggcg cccagctggg caggggtggg tgctctggcc tataaagccc 121 ccgaggccct gtggcctgca gaggcggtta tggacggcac catggaggac tccgaggcgg 181 tgcagagggc cacagcgctc atcgagcagc ggctggcaca ggaggaggag aatgagaaac 241 tccgaggaga cgcacgccag aagctgccca tggacttgct ggtgctggag gatgagaagc 301 accacggggc tcagagtgca gccctgcaga aggtgaaggg ccaagagcgc gtgcgcaaga 361 cgtccctgga cctgcggcgg gagatcatcg atgtgggcgg gatccagaac ctcatcgagc 421 tgcggaagaa acgcaagcag aagaagcggg acgctctggc cgcctcgcat gagccgcccc 481 cagagcccga ggagatcact ggccctgtgg atgaggagac cttcctgaaa gctgcggtgg 541 aggggaaaat gaaggtcatt gagaagttcc tggctgacgg ggggtcagcc gacacgtgcg 601 accagttccg tcggacagca ctgcaccgag cttccctgga aggccacatg gaaatcctgg 661 agaagcttct agataatggg gccactgtgg acttccagga tcggctggac tgcacagcca 721 tgcattgggc ctgccgcggg ggccacttag aggtggtgaa acttctgcaa agccatggag 781 cagacaccaa tgtgagggat aagctgctga gcaccccgct gcacgtggca gtccggacag 841 ggcaggtgga gattgtggag cactttctat ccctgggcct ggaaatcaat gccagagaca 901 gggaagggga tactgccctg catgacgctg tgaggctcaa ccgctacaaa atcatcaaac 961 tgctgctcct gcatggggct gacatgatga ccaagaacct ggcgggaaag accccgacgg 1021 acctggtgca gctctggcag gctgataccc ggcacgccct ggagcatcct gagccggggg 1081 ctgagcataa cgggctggag gggcctaatg atagtgggcg agagacccct cagcctgtgc 1141 cagcccagtt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1201 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1261 tcaacctctg gatta