Construct: ORF ccsbBroad304_08063

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012937.1_s304c1, BRDN0000398919
Derived from:
ccsbBroadEn_08063
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RSPH14 (27156)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08063
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27156 RSPH14 radial spoke head 14 homolog NM_014433.3 99.9% 100% 148T>C
2 human 27156 RSPH14 radial spoke head 14 homolog XM_011530149.2 87.3% 87.4% 148T>C;790_939del
3 human 27156 RSPH14 radial spoke head 14 homolog XM_011530150.1 86.4% 68.5% 148T>C;787_919del;1178_1206del
4 human 27156 RSPH14 radial spoke head 14 homolog XM_011530151.2 86.4% 68.5% 148T>C;787_919del;1178_1206del
5 human 27156 RSPH14 radial spoke head 14 homolog XM_011530152.2 86.4% 68.5% 148T>C;787_919del;1178_1206del
6 human 27156 RSPH14 radial spoke head 14 homolog XM_017028774.1 86.4% 68.5% 148T>C;787_919del;1178_1206del
7 human 27156 RSPH14 radial spoke head 14 homolog XM_011530154.2 69.6% 52% 0_1ins204;583_715del;974_1002del
8 human 27156 RSPH14 radial spoke head 14 homolog XM_011530155.2 52.3% 35.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1110
ORF length:
1044
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ccattcccag aactccttgg agcttcccat taacatcaat gccacccaga
121ttaccactgc ctatggccat cgggccctgc ccaagctgaa ggaggagctg cagtcagagg
181acctccagac gaggcagaaa gccctcatgg ccctgtgtga cctcatgcat gaccccgagt
241gtatctacaa ggccatgaac ataggctgta tggagaacct gaaagctttg ctgaaggata
301gcaacagtat ggtgcgcata aagaccaccg aggtgctcca catcacggca agccatagcg
361tgggcagata cgcctttcta gagcacgaca tcgtccttgc cctgtccttc ctgctgaatg
421accccagccc agtctgccgg gggaacctgt acaaggcata catgcagctg gtccaggtgc
481ctagaggggc ccaagagatc atcagcaaag gtctgatttc ctcactggta tggaagctgc
541aggtggaggt ggaggaggag gagttccagg agttcatcct ggacacactg gtcctctgcc
601tgcaggagga tgccaccgag gccctgggca gcaatgtggt gcttgtcctg aagcagaagc
661tcctcagcgc caaccagaac atccgcagca aggccgcccg tgcgctcctt aatgtcagca
721tatctcgaga gggcaagaaa caggtgtgtc attttgacgt catccccatc ctggtccatc
781tgctgaaaga cccagtggag catgtgaagt ctaacgctgc cggtgccctg atgttcgcca
841cagtgatcac tgaagggaag tatgcggccc tggaggcaca agccatcggc ctgctcctgg
901agctgctgca ctcccccatg accatagcgc gcctgaatgc caccaaggcc cttaccatgc
961tggcagaggc ccccgagggc cgcaaggccc tgcagacgca cgtgcccact ttccgtgcca
1021tggaggtgga gacttacgaa aagcctcaag tggccgaagc cttacagcgg gcagcccgga
1081tcgccatcag tgtcatcgag ttcaaaccct gcccaacttt cttgtacaaa gtggttggta
1141agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1201ggtggcgcgt taagtcgaca atcaacctct ggatta

Download FASTA (ORF) (Full)

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