Construct: ORF ccsbBroad304_08146

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013078.1_s304c1, BRDN0000462133
Derived from:
ccsbBroadEn_08146
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDZRN4 (29951)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08146
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29951 PDZRN4 PDZ domain containing ring ... NM_013377.4 99.9% 99.8% 2329A>C
2 human 29951 PDZRN4 PDZ domain containing ring ... NM_001164595.2 74.6% 72.3% (many diffs)
3 mouse 239618 Pdzrn4 PDZ domain containing RING ... NM_001164594.1 85.1% 88.4% (many diffs)
4 mouse 239618 Pdzrn4 PDZ domain containing RING ... NM_001164593.1 64.2% 65.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2403
ORF length:
2334
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggctgcaat ttgtgcactt ttcagaaaag agaggaacac tacaaactgc
121tgtatgaagt ttcccaggtc aatgggaagg atctttcaaa ggccactcat gaagaggcag
181tggaagcttt tcgcaatgcc aaggagccca ttgtggtgca ggtgttaagg cgaacacctc
241ttagtagacc agcctatggg atggcttcag aagtgcagct tatgaatgcc agcactcaga
301cggacatcac cttcgaacac atcatggctc tggccaagct tcgtccacct acccctccag
361tgccagacat ctgtccattc ctgctctcag acagctgcca ttctctacat ccaatggagc
421atgaatttta tgaggacaat gagtatattt ccagcttgcc tgctgatgca gacagaacag
481aagactttga atatgaggag gtcgagttgt gtcgtgttag cagtcaagag aagctgggcc
541tgacagtctg ttaccgaaca gatgatgaag aagacaccgg catttatgtc agcgaggttg
601acccaaatag cattgctgcc aaagacggcc ggattcgaga aggggatcgg attttgcaaa
661taaatgggga agatgtccag aatcgagaag aagcagtggc cttgctgtct aacgatgagt
721gtaagagaat cgtgctgctt gttgcaaggc cagagattca gctggatgaa ggctggctgg
781aagatgaaag gaatgaattc ttagaggagt taaacttgga gatgttggaa gaagagcata
841atgaagcaat gcagcccact gccaatgagg tggagcagcc aaaaaagcaa gaagaagaag
901aaggcacaac agacactgca acatcctcat ccaacaacca tgagaaggac agtggagtag
961gacgtacaga tgaaagcttg cgaaatgatg agagctcaga gcaggagaat gcagccgagg
1021accccaatag cacatctttg aagagcaaga gagacctggg gcagagccaa gacactctgg
1081gaagtgttga acttcagtac aatgagagcc tcgtatctgg tgaatacatt gactcagact
1141gcattggcaa cccagatgag gactgtgaaa gattcaggca gctcttggag ctcaaatgca
1201agattcgaaa tcatggagag tatgacctgt attactcaag cagcacaatt gaatgcaatc
1261aaggggagca agagggagtg gagcatgagc tacagttgct taatgaagaa ctgagaaaca
1321ttgagcttga gtgtcagaat atcatgcagg ctcacaggct ccagaaagtg acagaccagt
1381atggagacat ctggacattg catgatggag gattccggaa ttataacacc agcatagata
1441tgcaaagggg aaagctagat gacatcatgg agcatccaga aaagtctgac aaggacagtt
1501ctagtgctta caacacagct gagagctgca gaagtactcc gctcactgta gaccgttccc
1561ctgacagttc ccttccaagg gtgatcaacc tcaccaataa gaaaaacctg agaagcacaa
1621tggcagccac ccagtcctct tccggacaga gcagtaaaga gtcgacctcc accaaagcca
1681aaaccactga gcaaggttgt agcgctgaaa gcaaggagaa ggttttagaa ggcagcaagc
1741ttcctgatca agagaaggca gtcagcgaac acatccctta cctctctcct taccacagct
1801cctcatatag atatgcaaac atcccagcac acgcccggca ttatcaaagc tacatgcagt
1861taattcaaca gaaatctgca gtcgagtatg ctcagagtca gctcagcttg gtgagcatgt
1921gcaaggagtc tcagaagtgt tcagagccca agatggaatg gaaggtgaaa attaggagcg
1981acgggacacg gtacatcaca aagagacccg tgcgagaccg aatcctgaag gaacgtgcct
2041taaagatcaa ggaagagcgg agtggcatga ccacagacga tgacaccatg agcgagatga
2101aaatggggcg ctactggagc aaagaggaga gaaagcagca cctggttagg gccaaagagc
2161agcgccgtcg ccgtgagttc atgatgcgaa gcaggttaga gtgtctcaag gagagccctc
2221agagcggcag tgagggcaag aaggagatca atatcattga actgagtcac aaaaagatga
2281tgaaaaagag aaacaagaaa attttggaca actggatgac aatccaagaa ctgatgaccc
2341atggggccaa gtctccagat ggcacgagag tccataatgc cttcttgtcg gtgacccctg
2401tattgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
2461tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
2521tctggatta

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