Construct: ORF ccsbBroad304_08152

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007236.1_s304c1, BRDN0000398985
Derived from:
ccsbBroadEn_08152
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSAT1 (29968)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08152
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29968 PSAT1 phosphoserine aminotransfer... NM_058179.4 99.9% 100% 297T>G
2 human 29968 PSAT1 phosphoserine aminotransfer... NM_021154.5 87.4% 87.5% 297T>G;869_870ins138
3 mouse 107272 Psat1 phosphoserine aminotransfer... NM_177420.2 86.2% 92.4% (many diffs)
4 mouse 107272 Psat1 phosphoserine aminotransfer... NM_001205339.1 83.8% 85.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1176
ORF length:
1110
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga cgcccccagg caggtggtca actttgggcc tggtcccgcc aagctgccgc
121actcagtgtt gttagagata caaaaggaat tattagacta caaaggagtt ggcattagtg
181ttcttgaaat gagtcacagg tcatcagatt ttgccaagat tattaacaat acagagaatc
241ttgtgcggga attgctagct gttccagaca actataaggt gatttttctg caaggaggtg
301ggtgcggcca gttcagtgct gtccccttaa acctcattgg cttgaaagca ggaaggtgtg
361cggactatgt ggtgacagga gcttggtcag ctaaggccgc agaagaagcc aagaagtttg
421ggactataaa tatcgttcac cctaaacttg ggagttatac aaaaattcca gatccaagca
481cctggaacct caacccagat gcctcctacg tgtattattg cgcaaatgag acggtgcatg
541gtgtggagtt tgactttata cccgatgtca agggagcagt actggtttgt gacatgtcct
601caaacttcct gtccaagcca gtggatgttt ccaagtttgg tgtgattttt gctggtgccc
661agaagaatgt tggctctgct ggggtcaccg tggtgattgt ccgtgatgac ctgctggggt
721ttgccctccg agagtgcccc tcggtcctgg aatacaaggt gcaggctgga aacagctcct
781tgtacaacac gcctccatgt ttcagcatct acgtcatggg cttggttctg gagtggatta
841aaaacaatgg aggtgccgcg gccatggaga agcttagctc catcaaatct caaacaattt
901atgagattat tgataattct caaggattct acgtttgtcc agtggagccc caaaatagaa
961gcaagatgaa tattccattc cgcattggca atgccaaagg agatgatgct ttagaaaaaa
1021gatttcttga taaagctctt gaactcaata tgttgtcctt gaaagggcat aggtctgtgg
1081gaggcatccg ggcctctctg tataatgctg tcacaattga agacgttcag aagctggccg
1141ccttcatgaa aaaatttttg gagatgcatc agctatgccc aactttcttg tacaaagtgg
1201ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1261ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)