Transcript: Human NM_021154.5

Homo sapiens phosphoserine aminotransferase 1 (PSAT1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-09
Taxon:
Homo sapiens (human)
Gene:
PSAT1 (29968)
Length:
2068
CDS:
87..1061

Additional Resources:

NCBI RefSeq record:
NM_021154.5
NBCI Gene record:
PSAT1 (29968)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_021154.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000035264 GCCAAGAAGTTTGGGACTATA pLKO.1 429 CDS 100% 13.200 9.240 N PSAT1 n/a
2 TRCN0000291668 GCCAAGAAGTTTGGGACTATA pLKO_005 429 CDS 100% 13.200 9.240 N PSAT1 n/a
3 TRCN0000035267 CTCCATCAAATCTCAAACAAT pLKO.1 899 CDS 100% 5.625 3.938 N PSAT1 n/a
4 TRCN0000291670 CTCCATCAAATCTCAAACAAT pLKO_005 899 CDS 100% 5.625 3.938 N PSAT1 n/a
5 TRCN0000035265 CCAGACAACTATAAGGTGATT pLKO.1 285 CDS 100% 4.950 3.465 N PSAT1 n/a
6 TRCN0000291669 CCAGACAACTATAAGGTGATT pLKO_005 285 CDS 100% 4.950 3.465 N PSAT1 n/a
7 TRCN0000034630 GCCTCCATGTTTCAGCATCTA pLKO.1 812 CDS 100% 4.950 3.465 N LOC389173 n/a
8 TRCN0000035268 GTTTCCAAGTTTGGTGTGATT pLKO.1 648 CDS 100% 4.950 3.465 N PSAT1 n/a
9 TRCN0000291731 GTTTCCAAGTTTGGTGTGATT pLKO_005 648 CDS 100% 4.950 3.465 N PSAT1 n/a
10 TRCN0000035266 GCACTCAGTGTTGTTAGAGAT pLKO.1 140 CDS 100% 4.950 2.970 N PSAT1 n/a
11 TRCN0000291729 GCACTCAGTGTTGTTAGAGAT pLKO_005 140 CDS 100% 4.950 2.970 N PSAT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_021154.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491391 GAAAACGCAGAACTGTAAAGGTCG pLX_317 21.6% 87.5% 87.5% V5 (not translated due to prior stop codon) 869_870ins138 n/a
2 ccsbBroadEn_08153 pDONR223 100% 87.4% 87.2% None 118C>G;869_870ins138 n/a
3 ccsbBroad304_08153 pLX_304 0% 87.4% 87.2% V5 118C>G;869_870ins138 n/a
4 TRCN0000480450 AAAAAACTACCCTTACTTTTAGCG pLX_317 40.7% 87.4% 87.2% V5 118C>G;869_870ins138 n/a
5 ccsbBroadEn_08152 pDONR223 100% 87.4% 87.5% None 297T>G;869_870ins138 n/a
6 ccsbBroad304_08152 pLX_304 0% 87.4% 87.5% V5 297T>G;869_870ins138 n/a
7 TRCN0000471640 ACAGAACGGACCTCTGCTCTAATC pLX_317 45.3% 87.4% 87.5% V5 297T>G;869_870ins138 n/a
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