Construct: ORF ccsbBroad304_08322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016194.1_s304c1, BRDN0000402564
- Derived from:
- ccsbBroadEn_08322
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UFM1 (51569)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08322
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NM_016617.4 | 99.6% | 100% | 240T>C |
| 2 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NM_001286703.2 | 97.2% | 97.6% | 59_60insACTCAG;234T>C |
| 3 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NM_001286705.2 | 78.9% | 77.6% | (many diffs) |
| 4 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NM_001286706.2 | 78.9% | 77.6% | (many diffs) |
| 5 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NM_001286704.2 | 71.9% | 65.4% | (many diffs) |
| 6 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NR_104584.2 | 7.8% | (many diffs) | |
| 7 | human | 51569 | UFM1 | ubiquitin fold modifier 1 | NR_104585.2 | 6.7% | (many diffs) | |
| 8 | mouse | 67890 | Ufm1 | ubiquitin-fold modifier 1 | NM_026435.5 | 90.1% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 321
- ORF length:
- 255
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc gaaggtttcc tttaagatca cgctgacgtc ggacccacgg ctgccgtaca 121 aagtactcag tgttcctgaa agtacacctt tcacagcagt cttaaagttt gcagcagaag 181 aatttaaagt tcctgctgca acaagtgcaa ttattaccaa tgatggaata ggaataaatc 241 ctgcacagac tgctggaaat gtttttctaa aacatggttc agaactgcgg attattccta 301 gagaccgtgt tggaagttgt tacccaactt tcttgtacaa agtggttggt aagcctatcc 361 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 421 ttaagtcgac aatcaacctc tggatta