Construct: ORF ccsbBroad304_08347

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016034.1_s304c1, BRDN0000403797
Derived from:
ccsbBroadEn_08347
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GALNT7 (51809)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08347
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51809 GALNT7 polypeptide N-acetylgalacto... NM_017423.3 99.9% 100% 18G>A
2 human 51809 GALNT7 polypeptide N-acetylgalacto... XM_005263062.2 97% 97.5% (many diffs)
3 human 51809 GALNT7 polypeptide N-acetylgalacto... XM_017008292.2 96% 92.6% (many diffs)
4 human 51809 GALNT7 polypeptide N-acetylgalacto... XM_011532025.3 93.7% 93.6% 0_1ins123;2T>G
5 human 51809 GALNT7 polypeptide N-acetylgalacto... XM_017008293.1 93.1% 90.2% (many diffs)
6 mouse 108150 Galnt7 UDP-N-acetyl-alpha-D-galact... NM_144731.4 91.3% 95.1% (many diffs)
7 mouse 108150 Galnt7 UDP-N-acetyl-alpha-D-galact... NM_001167981.1 88.7% 93.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2037
ORF length:
1971
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag gctgaagatt ggattcatct tacgcagttt gctggtggtg ggaagcttcc
121tggggctagt ggtcctctgg tcttccctga ccccgcggcc ggacgaccca agcccgctga
181gcaggatgag ggaagacaga gatgtcaatg accccatgcc caaccgaggc ggcaatggac
241tagctcctgg ggaggacaga ttcaaacctg tggtaccatg gcctcatgtt gaaggagtag
301aagtggactt agagtctatt agaagaataa acaaggccaa aaatgaacaa gagcaccatg
361ctggaggaga ttcccagaaa gatatcatgc agaggcagta tctcacattt aagcctcaga
421cattcaccta ccatgatcct gtgcttcgcc cagggatcct cggtaacttt gaacccaaag
481aacctgagcc tcctggagtg gttggtggcc ctggagagaa agccaagcca ttggttttgg
541gaccagaatt caaacaagca attcaagcca gcattaaaga gtttggattt aacatggtgg
601caagtgacat gatctcactg gaccgcagcg tcaatgactt acgccaagaa gaatgcaagt
661attggcatta tgatgaaaac ttgctcactt cgagcgttgt cattgtcttc cataatgaag
721gatggtcaac cctcatgaga acagtccaca gtgtaattaa aaggactcca aggaaatatt
781tagcagaaat tgtgttaatt gacgatttca gtaataaaga acacttaaaa gaaaaactgg
841atgaatatat taagctgtgg aatggcctag tgaaggtatt tcgaaatgaa agaagggaag
901gtttaattca agcacgaagt attggtgctc agaaggctaa acttggacag gttttgatat
961accttgatgc ccactgtgag gtggcagtta actggtatgc accacttgta gctcccatat
1021ctaaggacag aaccatttgc actgtgccgc ttatagatgt cataaatggc aacacatatg
1081aaattatacc ccaagggggt ggtgatgaag atgggtatgc ccgaggagca tgggattgga
1141gtatgctctg gaaacgggtg cctctgaccc ctcaagagaa gagactgaga aagacaaaaa
1201ctgaaccgta tcggtcccca gccatggctg ggggattatt tgccattgaa cgagagttct
1261tctttgaatt gggtctctat gatccaggtc tccagatttg gggtggtgaa aactttgaga
1321tctcatacaa gatatggcag tgtggtggca aattattatt tgttccttgt tctcgtgttg
1381gacatatcta ccgtcttgag ggctggcaag gaaatcctcc gcccatttat gttgggtctt
1441ctccaactct gaagaattat gttagagttg tggaggtttg gtgggatgaa tataaagact
1501acttctatgc tagtcgtcct gaatcgcagg cattaccata tggggatata tcggagctga
1561aaaaatttcg agaagatcac aactgcaaaa gttttaagtg gttcatggaa gaaatagctt
1621atgatatcac ctcacactac cctttgccac ccaaaaatgt tgactgggga gaaatcagag
1681gcttcgaaac tgcttactgc attgatagca tgggaaaaac aaatggaggc tttgttgaac
1741taggaccctg ccacaggatg ggagggaatc agcttttcag aatcaatgaa gcaaatcaac
1801tcatgcagta tgaccagtgt ttgacaaagg gagctgatgg atcaaaagtt atgattacac
1861actgtaatct aaatgaattt aaggaatggc agtacttcaa gaacctgcac agatttactc
1921atattccttc aggaaagtgt ttagatcgct cagaggtcct gcatcaagta ttcatctcca
1981attgtgactc cagtaaaacg actcaaaaat gggaaatgaa taacatccat agtgtttacc
2041caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
2101ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
2161tta

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