Construct: ORF ccsbBroad304_08458
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002517.1_s304c1, BRDN0000402003
- Derived from:
- ccsbBroadEn_08458
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXO34 (55030)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08458
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55030 | FBXO34 | F-box protein 34 | NM_017943.4 | 99.8% | 99.7% | 600T>C;1409T>A;1598T>C |
| 2 | human | 55030 | FBXO34 | F-box protein 34 | NM_152231.1 | 99.8% | 99.7% | 600T>C;1409T>A;1598T>C |
| 3 | human | 55030 | FBXO34 | F-box protein 34 | XM_005267791.4 | 99.8% | 99.7% | 600T>C;1409T>A;1598T>C |
| 4 | human | 55030 | FBXO34 | F-box protein 34 | XM_006720185.3 | 99.8% | 99.7% | 600T>C;1409T>A;1598T>C |
| 5 | human | 55030 | FBXO34 | F-box protein 34 | XM_017021392.1 | 99.8% | 99.7% | 600T>C;1409T>A;1598T>C |
| 6 | human | 55030 | FBXO34 | F-box protein 34 | XM_017021393.2 | 99.8% | 99.7% | 600T>C;1409T>A;1598T>C |
| 7 | human | 55030 | FBXO34 | F-box protein 34 | XM_017021391.1 | 99.3% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2202
- ORF length:
- 2133
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcacctaaag ccatattgga agctccagaa gaaagagcac cccccggaag 121 tcagcaggga aacgcagaga actcctatga accaccaaaa ggctgtaaat gatgaaacat 181 gcaaagctag ccacataaca tcaagtgtct ttccttcagc ctctctcggt aaagcatcat 241 ctcgaaagcc atttgggatc ctttctccaa atgttctgtg cagtatgagt gggaagagtc 301 ctgtagagag cagcttgaat gttaaaacca aaaagaatgc accatctgca acgatccacc 361 agggcgaaga agaaggacca cttgatatct gggctgttgt gaaacctgga aataccaagg 421 aaaaaattgc attctttgca tcccaccagt gtagtaacag gataggatct atgaaaataa 481 aaagttcctg ggatattgat gggagagcta ctaagagaag gaaaaaatca ggggatctta 541 aaaaagccaa ggtacaggtg gaaaggatga gggaggttaa cagcaggtgc taccaacctg 601 agccttttgc atgtggcatt gagcactgtt ctgtgcacta tgtgagtgac agtggggatg 661 gagtctacgc tgggaggcct ctgtcagtta tacagatggt tgccttcttg gagcaaagag 721 ccagtgctct gctagctagc tgttcaaaaa actgcacaaa ctcacctgca attgtgaggt 781 tttctggcca atccagaggt gtgcctgcag tgtctgagtc ctattctgcc ccaggagctt 841 gtgaagaacc cacagaaagg ggaaatcttg aggttggtga accacagagc gaaccagtcc 901 gtgtccttga catggtagcc aagttggagt ctgagtgcct gaagcggcag ggccagcgtg 961 agcctgggag cctctcaagg aataacagct tccgtcgaaa tgtgggcaga gtattgcttg 1021 caaatagcac tcaggctgat gaaggcaaaa caaagaaagg cgtcttggag gcacctgaca 1081 ctcaggtgaa tcctgtgggg tctgtatctg tggattgtgg cccttcaaga gctgatcgtt 1141 gttctcctaa ggaggaccag gcctgggacg gtgcttctca ggactgcccc ccattgccag 1201 caggagtgag tttccacata gacagtgcag agttagagcc gggttcgcaa actgccgtga 1261 aaaacagcaa cagatatgat gtggaaatga cagatgaact cgttgggtta cctttttcct 1321 ctcataccta ttcccaagcc tctgaattgc ccacagatgc tgttgattgt atgagcagag 1381 agcttgtgtc ccttactagc cgaaatcctg atcaaagaaa agaatctttg tgcattagta 1441 tcactgtgtc caaggtagac aaagaccagc cttccaattt aaactcctgt gaagacccag 1501 ttccagggat gttgtttttt ttgccacctg gtcagcactt gtcagactat tcccagttga 1561 atgaaagcac aacaaaagag tcttcagagg ccagccagct tgaagatgct gctgggggtg 1621 acagtgcatc tgaggaaaaa agtgggtctg ctgagccatt tgtaccgcca gcctcttctg 1681 tggaaagtac attaccagtg cttgaggcat ccagttggaa gaagcaggtg tcgcatgact 1741 tcctggagac caggtttaaa atccagcagc ttttggagcc tcagcagtac atggcttttc 1801 tgccccacca cattatggta aaaatcttca ggttacttcc caccaagagt ttagtggccc 1861 ttaaatgtac ctgctgctat ttcaagttta tcattgagta ctacaatatc aggccagcag 1921 attctcgctg ggttcgagat ccacgctata gagaggatcc ttgcaaacag tgcaagaaaa 1981 agtatgtgaa aggggatgtg tccctgtgcc gatggcaccc caagccctat tgccaggcat 2041 tgccctatgg gccagggtat tggatgtgct gccaccggtc tcagaaagga ttccctggct 2101 gtaagctggg gcttcatgac aatcactggg ttcctgcctg ccacagcttt aatcgggcaa 2161 tccataagaa agcaaaaggg actgaagctg aagaggaata cttgccaact ttcttgtaca 2221 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2281 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta