Construct: ORF ccsbBroad304_08460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015312.1_s304c1, BRDN0000407207
- Derived from:
- ccsbBroadEn_08460
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOCOS (55034)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08460
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55034 | MOCOS | molybdenum cofactor sulfurase | NM_017947.4 | 99.7% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2730
- ORF length:
- 2664
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cggcgcggcg gcggagtcag ggcgggagct gtggaccttc gcgggttccc 121 gggacccgag cgcaccgcgg ctagcctacg gctacggccc gggcagcctg cgcgagctgc 181 gggcgcgcga gttcagccgc ctggcaggaa ctgtctatct tgaccatgca ggtgccacct 241 tgttctccca gagccagctc gaaagcttca ctagtgatct catggaaaac acttatggta 301 atcctcacag ccagaacatc agcagcaagc tcacccatga cactgtggag caggtgcgct 361 acagaatcct ggcgcacttc cacaccaccg cagaagacta cactgtgatc ttcactgccg 421 ggagcacggc tgctctcaaa ctggtggcag aggcctttcc atgggtgtcc cagggcccag 481 agagcagtgg gagtcgcttc tgttacctca ccgacagcca cacctccgta gtgggtatga 541 ggaacgtgac catggctata aatgtcatat ccatcccggt caggccagag gacctgtggt 601 ctgcagagga acgtggtgct tcagccagca acccagactg ccagctgccg catctcttct 661 gctacccagc tcagagtaac ttttctggag tcagataccc cctgtcctgg atagaagagg 721 tcaagtctgg gcggttgcgc cctgtgagca cgcctgggaa gtggtttgtg ctgctggatg 781 cagcctccta cgtgagcacc tcgcctttgg acctgtcagc tcaccaggcc gactttgtcc 841 ccatctcctt ctataagatc ttcgggtttc ctacaggcct gggcgctctg ctggtccata 901 atcgtgcggc tcctctactg aggaagacct actttggagg agggacagcc tctgcgtacc 961 tagcaggaga agacttctac atcccgaggc agtcggtagc tcagaggttt gaagatggca 1021 ccatctcatt ccttgatgtt atcgcgctaa aacatggatt tgacacccta gagcgcctca 1081 caggtggaat ggagaatata aagcagcaca ccttcacctt ggctcagtat acctacatgg 1141 ccctgtcctc tctccagtac cccaatggag cccctgtggt gcggatttac agcgattctg 1201 agttcagcag ccctgaggtt cagggcccga tcatcaattt taatgtgctg gatgacaaag 1261 ggaacatcat tggttactcc caggtggaca aaatggccag tctttacaac atccacctgc 1321 gaactggctg cttctgtaac actggggcct gccagaggca cctgggcata agcaacgaga 1381 tggtcaggaa gcattttcag gctggtcatg tctgtgggga caatatggac ctcatagatg 1441 ggcagcccac aggatctgtg aggatttcat ttggatacat gtcgacgctg gatgatgtcc 1501 aggcctttct taggttcatc atagacactc gcctgcactc atcaggggac tggcctgtcc 1561 ctcaggccca tgctgacacc ggggagactg gagccccatc agcagacagc caggctgatg 1621 ttatacctgc tgtcatgggc agacgtagcc tctcgcctca ggaagatgcc ctcacaggct 1681 ccagggtttg gaacaactcg tctactgtga atgctgtgcc tgtggcccca cctgtgtgtg 1741 atgtcgccag aacccagccg actccttcag agaaagctgc aggagtcctg gagggggccc 1801 ttgggccaca tgttgtcact aacctttatc tctatccaat caaatcctgt gctgcatttg 1861 aggtgaccag gtggcctgta ggaaaccaag ggctgctata tgaccggagc tggatggttg 1921 tgaatcacaa tggtgtttgc ctgagtcaga agcaggaacc ccggctctgc ctgatccagc 1981 ccttcatcga cttgcggcaa aggatcatgg tcatcaaagc caaagggatg gagcctatag 2041 aggtgcctct tgaggaaaat agtgaacgga ctcagattcg ccaaagcagg gtctgtgctg 2101 acagagtaag tacttatgat tgtggagaaa aaatttcaag ctggttgtca acattttttg 2161 gccgtccttg tcatttgatc aaacaaagtt caaactctca aaggaatgca aagaagaaac 2221 atggaaaaga tcaacttcct ggtacaatgg ccaccctttc tctggtgaat gaggcacagt 2281 atctgctgat caacacatcc agtattttgg aacttcaccg gcaactaaac accagtgatg 2341 agaatggaaa ggaggaatta ttctcactga aggatctcag cttgcgtttt cgtgccaata 2401 ttattatcaa tggaaaaagg gcttttgaag aagagaaatg ggatgagatt tcaattggct 2461 ctttgcgttt ccaggttttg gggccttgtc acagatgcca gatgatttgc atcgaccagc 2521 aaactgggca acgaaaccag catgttttcc aaaaactttc tgagagtcgt gaaacaaagg 2581 tgaactttgg catgtacctg atgcatgcat cattggattt atcctcccca tgtttcctgt 2641 ctgtaggatc tcaggtgctc cctgtgttga aagagaatgt ggaaggtcat gatttacctg 2701 catctgagaa acaccaggtt gttacctcct acccaacttt cttgtacaaa gtggttggta 2761 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2821 ggtggcgcgt taagtcgaca atcaacctct ggatta