Construct: ORF ccsbBroad304_08465

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013716.1_s304c1, BRDN0000407669
Derived from:
ccsbBroadEn_08465
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC52A1 (55065)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08465
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55065 SLC52A1 solute carrier family 52 me... NM_001104577.1 99.8% 99.5% 209A>G;812C>T
2 human 55065 SLC52A1 solute carrier family 52 me... NM_017986.4 99.8% 99.5% 209A>G;812C>T
3 human 55065 SLC52A1 solute carrier family 52 me... XM_011523951.2 99.8% 99.5% 209A>G;812C>T
4 human 79581 SLC52A2 solute carrier family 52 me... NM_001253815.2 86.7% 86.1% (many diffs)
5 human 79581 SLC52A2 solute carrier family 52 me... NM_001253816.2 86.7% 86.1% (many diffs)
6 human 79581 SLC52A2 solute carrier family 52 me... NM_001363118.2 86.7% 86.1% (many diffs)
7 human 79581 SLC52A2 solute carrier family 52 me... NM_001363120.2 86.7% 86.1% (many diffs)
8 human 79581 SLC52A2 solute carrier family 52 me... NM_001363121.2 86.7% 86.1% (many diffs)
9 human 79581 SLC52A2 solute carrier family 52 me... NM_024531.5 86.7% 86.1% (many diffs)
10 human 79581 SLC52A2 solute carrier family 52 me... XM_011517300.2 69.1% 68.5% (many diffs)
11 human 79581 SLC52A2 solute carrier family 52 me... NM_001363122.2 54.1% 53.1% (many diffs)
12 human 79581 SLC52A2 solute carrier family 52 me... XM_017013821.1 54.1% 53.1% (many diffs)
13 human 79581 SLC52A2 solute carrier family 52 me... XM_017013822.1 54.1% 53.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1410
ORF length:
1344
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agcacccacg ctgggccgtc tggtgctgac ccacctgctg gtggcccttt
121ttggcatggg ctcctgggct gctgtgaacg ggatctgggt ggagctgcct gtggtggtaa
181aagaccttcc agagggttgg agcctcccct catacctctc tgtggttgtg gcgctgggaa
241acctgggtct gctggtggtg accctgtgga ggcggctggc cccgggcaag ggcgagcagg
301tccccatcca ggtggtacag gtgctgagtg tagtgggcac agccctgctg gcccctctgt
361ggcaccacgt ggccccagtg gcagggcagc tccactctgt ggccttccta actctggcct
421tggtgttggc aatggcctgt tgtacctcta atgtcacttt cctgcccttc ctgagccacc
481tgccacctcc tttcttacgg tctttcttcc tgggtcaggg tctcagtgcc ctactcccct
541gtgtgctggc cctagtgcaa ggtgtgggcc gcctcgagtg cccaccagcg cccaccaatg
601gcacctctgg gcctcccctc gacttccctg agcgttttcc tgccagcacc ttcttctggg
661cactgactgc ccttctggtc acttcagctg ccgccttccg gggtctcctg ttgctgttgc
721catcactacc ctctgtaacc acagggggct cagggcctga acttcaactg ggatccccag
781gagcagagga ggaagagaag gaggaagaag aggctttgcc attgcaggag ccaccgagcc
841aggcagcagg caccatccct ggcccagacc ctgaggtcca tcagctgttc tcagcccatg
901gtgccttcct gctgggcctg atggccttca ccagtgccgt gaccaatggc gtgctgcctt
961ctgtgcagag cttttcctgt ttgccctatg ggcgcctggc ctaccacctg gctgtggtgc
1021tgggcagtgc cgccaacccc cttgcctgct tcctggccat gggcgtgctg tgcaggtccc
1081tggcagggct ggttggtctt tctctgctgg gcatgctctt tggggcctac ctgatggcac
1141tggcaatcct gagcccctgc ccacccctgg tgggcaccac tgcaggggtg gtccttgtgg
1201tgctgtcgtg ggtgctgtgt ctgtgtgtgt tctcatatgt gaaggtggct gcaagctccc
1261tgctgcatgg tgggggtcgg ccggcattgc tggcagctgg tgtggccatc caagtgggct
1321ccctgcttgg tgccggtgcc atgttccctc ccaccagcat ctaccacgtg tttcaaagca
1381gaaaggactg tgtagacccc tgtggcccct gcccaacttt cttgtacaaa gtggttggta
1441agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1501ggtggcgcgt taagtcgaca atcaacctct ggatta

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