Construct: ORF ccsbBroad304_08518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017453.1_s304c1, BRDN0000406937
- Derived from:
- ccsbBroadEn_08518
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ABCF3 (55324)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08518
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55324 | ABCF3 | ATP binding cassette subfam... | NM_018358.2 | 99.9% | 100% | 793C>T |
| 2 | human | 55324 | ABCF3 | ATP binding cassette subfam... | NM_001351298.1 | 99.1% | 99.1% | 202_203insTGTACAACACTCTGCGTC;775C>T |
| 3 | human | 55324 | ABCF3 | ATP binding cassette subfam... | NM_001351300.1 | 68.7% | 68.8% | 0_1ins663;130C>T |
| 4 | human | 55324 | ABCF3 | ATP binding cassette subfam... | XM_024453624.1 | 68.7% | 68.8% | 0_1ins663;130C>T |
| 5 | human | 55324 | ABCF3 | ATP binding cassette subfam... | XM_024453625.1 | 68.7% | 68.8% | 0_1ins663;130C>T |
| 6 | human | 55324 | ABCF3 | ATP binding cassette subfam... | NM_001351299.1 | 65.1% | 62.3% | 0_1ins736;57C>T;99_105delAGCCACA |
| 7 | mouse | 27406 | Abcf3 | ATP-binding cassette, sub-f... | NM_013852.2 | 88.4% | 96.8% | (many diffs) |
| 8 | mouse | 27406 | Abcf3 | ATP-binding cassette, sub-f... | XR_001781829.1 | 61.1% | (many diffs) | |
| 9 | mouse | 27406 | Abcf3 | ATP-binding cassette, sub-f... | XR_384567.3 | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2193
- ORF length:
- 2127
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgacatggc gacttgcgcc gaaatcctgc ggagcgagtt ccccgaaatt gacggacaag 121 tcttcgacta cgtgaccggc gtcttgcaca gcggcagcgc ggacttcgag tctgtggatg 181 acctggtgga agctgtaggg gaactattgc aagaggtgtc cggggacagc aaggatgacg 241 cgggcatcag ggccgtgtgc cagcgcatgt acaacactct gcgtctggct gagccacaaa 301 gccagggaaa tagccaggtg ctactggacg cccctatcca gttgtcaaag ataacggaga 361 actacgactg tggaaccaaa cttccaggac tgctaaagag ggaacagtcc tcgacagtga 421 atgcaaagaa gttagagaag gccgaggctc gacttaaggc aaagcaggag aagcgctcag 481 agaaggacac gctcaagacc agcaaccctc tagtcttaga agaggcatca gccagccagg 541 caggcagcag aaaggagagt cggttggaat catctggcaa gaacaaatcc tatgatgtgc 601 gaattgagaa ctttgatgtg tcttttggcg atagagtact gctggctgga gcggatgtga 661 acctggcatg gggccgccgt tacgggctgg tggggcggaa tgggttgggg aagacaacgt 721 tactgaagat gctggccacc cggagtctgc gggttccagc ccacatttcc ctgctgcacg 781 ttgagcaaga ggttgctgga gatgacactc ctgccctgca gagtgtgctg gagagtgaca 841 gtgtgcgaga ggatttgtta cggagggagc gggagctcac tgcccagatt gctgctggca 901 gggcggaggg ctctgaagct gcagagctgg cagaaatcta tgccaaactg gaggagattg 961 aggctgacaa ggcacctgcc agggcatcag tcattctcgc tgggcttggc tttaccccta 1021 aaatgcagca gcagcccacc cgggagttct caggtggctg gaggatgagg ctggccctgg 1081 cccgggccct ctttgctagg ccagatcttc tgctgttaga tgaacctaca aacatgctgg 1141 atgtcagggc catcctgtgg ctggagaatt acctgcagac gtggccctcc accatcctag 1201 tcgtctccca cgaccgcaac ttcttgaatg ccatcgccac agacatcatc cacctgcaca 1261 gccagcggct agatggttac cggggagact ttgagacctt catcaagagt aagcaggagc 1321 ggctgctcaa ccagcagcgt gaatatgagg cgcagcagca gtatcgccag cacatccagg 1381 ttttcattga ccggtttcgc tacaatgcca acagggcctc tcaagtgcag agtaaactca 1441 agatgctgga gaagctgcct gagctgaagc ctgtggacaa ggaatcagag gtcgtaatga 1501 agttccctga tgggtttgag aagttctcgc cgccaattct gcagctagat gaggtggatt 1561 tctactacga tccgaagcac gtcatcttca gtcgcctctc tgtgtctgct gatctcgagt 1621 ctcgcatctg tgtggttgga gagaatgggg ctgggaagtc taccatgctg aagctgcttt 1681 tgggggacct ggcacctgtt cggggcatca gacacgctca caggaatctg aagattggct 1741 atttcagcca gcaccatgtg gagcagctgg acctaaacgt cagtgctgtg gaactgctgg 1801 cacgcaagtt tcctgggcgg cctgaggagg agtaccgtca ccagctgggt cggtatggca 1861 tctccggaga actggccatg cgtcctcttg ccagcctgtc tgggggccag aagagccgag 1921 tggcctttgc tcagatgact atgccctgcc ccaacttcta cattctggat gaacccacaa 1981 accacctgga catggagacc attgaggctc tgggccgtgc cctcaacaat ttcaggggtg 2041 gtgtgattct ggtgtcccac gatgagcgct ttatcaggct ggtgtgccgg gagttgtggg 2101 tatgcgaagg aggcggcgtc acccgtgtgg aaggaggatt tgaccagtac cgcgccctcc 2161 tccaggaaca gttccgccgc gaaggcttcc tctagccaac tttcttgtac aaagtggttg 2221 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 2281 accggtggcg cgttaagtcg acaatcaacc tctggatta