Construct: ORF ccsbBroad304_08549

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003378.1_s304c1, BRDN0000462070
Derived from:
ccsbBroadEn_08549
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ZCCHC8 (55596)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08549
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55596 ZCCHC8 zinc finger CCHC-type conta... NM_017612.5 99.9% 99.7% 977C>T;1498T>C
2 human 55596 ZCCHC8 zinc finger CCHC-type conta... NM_001350935.2 89% 88.8% 501_502ins231;746C>T;1267T>C
3 human 55596 ZCCHC8 zinc finger CCHC-type conta... XM_017019606.1 86.1% 85% (many diffs)
4 human 55596 ZCCHC8 zinc finger CCHC-type conta... NM_001350936.2 85.9% 85.7% 0_1ins297;680C>T;1201T>C
5 human 55596 ZCCHC8 zinc finger CCHC-type conta... NM_001350937.2 66.2% 66% 0_1ins714;263C>T;784T>C
6 human 55596 ZCCHC8 zinc finger CCHC-type conta... NM_001350938.2 66.2% 66% 0_1ins714;263C>T;784T>C
7 human 55596 ZCCHC8 zinc finger CCHC-type conta... XR_944614.3 66.1% (many diffs)
8 human 55596 ZCCHC8 zinc finger CCHC-type conta... XM_024449044.1 63.4% 63.2% 0_1ins774;203C>T;724T>C
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2187
ORF length:
2121
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cgcagaggtg tattttggcg atctagagct cttcgagccg ttcgaccacc
121cagaggagtc gattccgaag cccgttcaca ctcgcttcaa ggacgacgac ggcgacgagg
181aggacgaaaa tggggtcggc gacgcggagc tacgggagcg gcttcggcag tgcgaggaga
241ccatcgagca gctccgcgcc gagaatcaag aacttaaacg aaaattgaac attctgactc
301gaccgagtgg aatattggtg aacgatacta agttagatgg acctatatta cagattctat
361tcatgaacaa tgctatttca aagcaatatc atcaagaaat agaggaattt gtatcaaatt
421tagtaaaaag atttgaggaa cagcagaaaa atgatgtgga aaagacttcc tttaatcttt
481tgccccagcc atccagtatt gtgctagagg aggaccacaa agtggaagag tcctgtgcca
541ttaaaaacaa caaggaagct ttcagtgttg taggaagtgt cctgtatttt actaattttt
601gccttgataa attggggcaa ccgcttctaa atgaaaaccc tcagctttcc gaaggatggg
661aaatacccaa gtaccatcaa gtcttcagcc acattgtttc tctagaaggg caagaaatac
721aagtaaaggc aaaaaggcca aagcctcact gtttcaattg tggttctgaa gaacaccaaa
781tgaaagattg cccaatgcct cggaatgctg ctcgaataag tgaaaagaga aaagagtata
841tggatgcctg tggagaagca aacaatcaga atttccagca gcgataccac gcagaagaag
901tagaagaaag atttggaaga ttcaagccag gagttattag tgaggaactt caagatgcac
961taggtgtgac agacaagagt cttccacctt ttatctatcg gatgcgccag ctagggtacc
1021caccagggtg gctcaaagag gttgaattgg agaattcggg gcttgcactc tatgatggaa
1081aagatggcac tgatggggaa acagaagttg gagaaataca acagaataaa agtgtcactt
1141acgatctctc aaaattggtc aactatcctg gttttaatat atctactccc agaggaattc
1201cagacgaatg gaggatcttt ggttccatac caatgcaggc atgtcagcag aaggatgtgt
1261ttgccaatta ccttacttct aacttccaag cgccaggtgt gaagtctggc aacaagaggt
1321cttcatctca ctctagccca ggtagtccaa agaagcagaa gaatgaaagc aactcagcgg
1381gatctcccgc cgacatggag ctcgattcag atatggaggt accacatggt tctcagagca
1441gcgaaagttt tcagtttcaa ccaccattac ctcctgacac tcctccactc ccccggggaa
1501ctcctccacc cgtcttcacc cctccactcc caaagggcac cccgccgctg actcccagtg
1561acccacccca gaccagaaca gcatctggag ctgtggatga ggacgcactg actctagaag
1621aacttgaaga acagcagagg cggatctggg cagctcttga gcaggccgag agcgtaaaca
1681gcgactccga cgttcctgtg gacacacctt taactggcaa ttccgttgcc tcatcacctt
1741gtccaaatga gctagacctc cctgtcccgg agggaaaaac atctgaaaag cagacgctgg
1801atgagcctga ggtaccagag atttttacaa agaaatcaga agctggacat gcctccagtc
1861cagactctga ggtgacatca ctttgtcaga aggaaaaagc agagttggct ccggtaaaca
1921ctgaaggtgc ccttcttgat aatggcagtg tcgtaccaaa ctgtgacatc agcaatgggg
1981gcagccagaa gctctttcct gcagacacca gtccttcaac ggccactaaa attcatagcc
2041ctatacctga catgagcaaa tttgcaactg gaatcacgcc atttgaattt gagaatatgg
2101cagaatctac tggaatgtac ctcaggataa gaagcttgtt aaagaactca ccccgaaacc
2161agcagaaaaa caaaaaggcc tctgaatacc caactttctt gtacaaagtg gttggtaagc
2221ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
2281ggcgcgttaa gtcgacaatc aacctctgga tta

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