Construct: ORF ccsbBroad304_08585

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006699.1_s304c1, BRDN0000461608
Derived from:
ccsbBroadEn_08585
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RIOK2 (55781)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08585
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55781 RIOK2 RIO kinase 2 NM_018343.3 99.8% 99.6% 430C>T;1636G>C
2 human 55781 RIOK2 RIO kinase 2 NM_001159749.2 85.3% 84.6% (many diffs)
3 human 55781 RIOK2 RIO kinase 2 XM_017009628.1 66% 65.9% 0_1ins561;1075G>C
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1722
ORF length:
1656
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg gaaagtgaat gtggccaagt tgcgttacat gagccgagat gacttcaggg
121tcttgaccgc ggttgaaatg ggcatgaaga accatgaaat tgttcccggc agtttgattg
181cttctatagc cagccttaaa catggtggct gtaataaagt tttaagagaa ttagtgaaac
241ataaactcat agcttgggag cgtaccaaaa ctgtccaggg ctatcggttg acaaatgcag
301gatatgatta cctagctttg aaaacacttt cttctaggca agtagttgag tctgttggaa
361accagatggg tgttggcaaa gaatcagata tttacattgt tgcaaatgaa gaaggacaac
421aatttgcatt aaagcttcac agactaggaa gaacctcgtt tcgaaatttg aaaaacaaac
481gcgattatca taaatatagg cacaatgtgt catggctata tttatctcgt ctctctgcca
541tgaaggaatt tgcctatatg aaggcattgt atgagaggaa atttccagtt ccaaagccaa
601ttgattacaa tcgtcatgca gtggtcatgg aactcataaa tggttatcca ctatgtcaga
661tacaccatgt tgaagatcct gcatcagtat atgatgaagc tatggaacta attgtcaaac
721ttgcaaatca tgggctgatt catggagatt ttaatgaatt taatctcatt ttggatgaaa
781gtgaccatat caccatgatt gattttccac agatggtttc aacttctcat cccaatgctg
841agtggtattt tgacagagat gttaaatgca ttaaagattt ctttatgaaa cgtttcagct
901acgaaagtga gctttttcca acttttaagg atatcaggag agaagacact cttgatgtgg
961aggtttctgc cagtggctac acaaaggaaa tgcaggcaga tgatgaactg cttcatccat
1021taggtccaga tgataaaaat attgaaacaa aagagggatc tgaattctca ttttcagatg
1081gagaagtggc agaaaaagca gaggtttacg ggtcagaaaa tgaaagtgaa cggaactgtc
1141tagaagaatc agagggctgc tattgcagat catctggaga ccctgaacaa ataaaggaag
1201acagtttatc agaagagagt gctgatgcac ggagttttga aatgactgaa ttcaatcaag
1261ctttagaaga aataaaaggg caggttgttg aaaacaactc tgtaactgaa ttttctgagg
1321agaaaaacag aactgaaaat tacaacaggc aagatggtca gagagttcaa ggaggagtcc
1381ctgctggctc tgacgagtat gaagatgaat gccctcatct aattgccttg tcgtcattaa
1441atagagaatt caggcctttc agagatgaag aaaatgtggg agctatgaat cagtatagaa
1501caagaactct gagtatcact tcttcaggca gtgctgtaag ctgttcaaca attcctccag
1561aactggtgaa acagaaggtg aaacgtcagt tgacaaaaca gcaaaaatca gctgtcagac
1621gtcgattgca gaaaggagaa gcaaatatat ttaccaagca acgtagggaa aacatgcaaa
1681atatcaaatc aagtttggaa ccagccagct tttggggaga atacccaact ttcttgtaca
1741aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1801ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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