Construct: ORF ccsbBroad304_08629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004289.1_s304c1, BRDN0000401521
- Derived from:
- ccsbBroadEn_08629
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHA12 (56137)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08629
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56137 | PCDHA12 | protocadherin alpha 12 | NM_031864.3 | 99.9% | 100% | 2280T>C |
| 2 | human | 56137 | PCDHA12 | protocadherin alpha 12 | NM_018903.4 | 83.9% | 83.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2445
- ORF length:
- 2376
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgattatc ggaccaagag gcccgggatc ccagcgtctg ctgctctcgc 121 ttctgctcct tgcagcctgg gaggtgggga gcggccagct ccactactcc gtctacgagg 181 aggccaaaca cggcaccttc gtgggccgca tcgctcagga cctggggctg gagctggcgg 241 agctggtgcc gcgcctgttc cgggtggcgt ccaaaagaca cggggacctt ctggaggtaa 301 atctgcagaa tggcattttg tttgtgaatt ctcggatcga ccgcgagaag ctgtgcgggc 361 ggagcgcgga gtgcagtatc cacctggagg tgatcgtgga caggccgctg caggttttcc 421 atgtggacgt ggaggtgaag gacattaacg acaacccgcc ggtgttcaga gaaagggaac 481 aaaaggtacc tgtttctgaa tctgcgcctc tggactctca ttttcctcta gagggcgctt 541 ctgatgcgga tatcggcgta aactctcttt tgacctatgc gttaagtcta aatgagaatt 601 ttgagcttaa aataaaaaca aaaaaagata aaagtatatt gcctgaatta gttcttcgga 661 agttattgga cagagagcaa acgccaaaac tcaatttatt gctgatggta atcgatggcg 721 gtaaaccaga actaacaggg tctgtccaga ttcaaataac cgtcctggat gtgaatgaca 781 atggtccggc gtttgataag cccagctata aagtagtgtt gtctgaaaat gtccaaaacg 841 acacaagagt gatccaacta aatgcttccg atccagacga aggacttaat ggagaaattt 901 cctatgggat caaaatgatt ttgccagtga gtgagaaatg tatgttttca ataaatccag 961 acacaggtga aattagaatt tatggtgaac tggattttga agagaataat gcctatgaaa 1021 ttcaggttaa cgccattgat aaagggattc cttccatggc aggtcacagc atggtcctgg 1081 tggaagttct ggacgtgaat gacaatgtcc ctgaagtaat ggttacttca ctgtcgctcc 1141 ctgtgcaaga ggatgctcag gtgggtaccg tcattgccct gattagcgtg tcggatcgtg 1201 actctggagc caatggacag gtcatctgct cactgacacc tcatgttccc ttcaagctgg 1261 tgtccaccta caagaattac tactcgttgg tgctggacag cgccctggac cgcgagagcg 1321 tgtcggccta tgagctggtg gtgactgcgc gggatggggg ctcgccttcg ctgtgggcca 1381 cggctagagt gtccgtggag gtggccgacg tgaacgacaa tgcgcctgcg ttcgcgcagc 1441 ccgagtacac agtgttcgtg aaggagaaca acccgccggg ctgccacatc ttcacggtgt 1501 cggcatggga cgcggacgcg cagaagaacg cgctggtgtc ctactcgctg gtggagcggc 1561 gggtgggcga gcacgcactg tcgagctacg tgtcggtgca cgcggagagc ggcaaggtgt 1621 acgcgctgca gccgctagac cacgaggagc tggagctgct gcagttccag gtgagcgcgc 1681 gcgacgccgg cgtgccgcct ctgggcagca acgtgacgct gcaggtgttc gtgctggacg 1741 agaacgacaa cgcgccggca ctgctggcga ctccggctgg cagcgcagga ggcgcagtta 1801 gcgagttggt accgcggtcg gtgggtgcgg gccacgtggt ggcgaaagtg cgcgcggtgg 1861 acgctgactc cggctataac gcttggctgt cctacgagtt gcaaccggcg gcggtcggcg 1921 cgcacatccc gttccacgtg gggctgtaca ctggcgagat cagcacgaca cgcatcctgg 1981 atgaggcgga cgctccgcgc caccgcctgc tggtgctggt gaaggaccac ggtgagcccg 2041 cgctgacgtc cacggccacg gtgctggtgt cgctggtgga gaacggccag gccccaaaga 2101 cgtcgtcgcg ggcctcagtg ggcgctgtgg atcccgaagc ggctctggtg gatattaacg 2161 tgtacctcat catcgccatc tgtgcggtgt ccagcctgct ggtgctcacg ctgctgctgt 2221 acactgcgct gcgttgctca gcgccgccca ccgtgagccg gtgcgcgccg ggcaagccca 2281 cgctggtgtg ctccagcgcc gtggggagtt ggtcttactc gcagcagagg aggcagaggg 2341 tgtgctccgc agagagcccg cccaagacgg acctcatggc cttcagccca agccttcagc 2401 tgtctcgaga agattgttta aatcctccca gtgaagtaag ttatttgcca actttcttgt 2461 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2521 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a