Construct: ORF ccsbBroad304_08670
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014793.1_s304c1, BRDN0000398689
- Derived from:
- ccsbBroadEn_08670
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUSP22 (56940)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08670
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56940 | DUSP22 | dual specificity phosphatas... | XM_017011062.1 | 65% | 65.3% | 1_129del;336G>A |
| 2 | human | 56940 | DUSP22 | dual specificity phosphatas... | XM_011514757.2 | 51% | 47.9% | (many diffs) |
| 3 | human | 56940 | DUSP22 | dual specificity phosphatas... | NM_020185.4 | 43.8% | 44% | 1_309del;516G>A |
| 4 | human | 56940 | DUSP22 | dual specificity phosphatas... | NM_001286555.1 | 36.2% | 33.9% | (many diffs) |
| 5 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104475.1 | 6.7% | (many diffs) | |
| 6 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104474.1 | 6.5% | (many diffs) | |
| 7 | human | 56940 | DUSP22 | dual specificity phosphatas... | NR_104473.1 | 6.5% | (many diffs) | |
| 8 | mouse | 105352 | Dusp22 | dual specificity phosphatas... | NM_134068.3 | 38.9% | 41.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 309
- ORF length:
- 243
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac cgtcactgac tttggctggg aggatgccct gcacaccgtg cgtgctggga 121 gatcctgtgc caaccccaac gtgggcttcc agagacagct ccaggagttt gagaagcatg 181 aggtccatca gtatcggcag tggctgaagg aagaatatgg agagagccct ttgcaggatg 241 cagaagaagc caaaaacatt ctggccgctc caggaattct gaagttctgg gcctttctca 301 gaagactgta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 361 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 421 tcaacctctg gatta