Construct: ORF ccsbBroad304_08706
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018023.1_s304c1, BRDN0000462031
- Derived from:
- ccsbBroadEn_08706
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGL3 (57139)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08706
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57139 | RGL3 | ral guanine nucleotide diss... | NM_001035223.4 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 57139 | RGL3 | ral guanine nucleotide diss... | NM_001161616.3 | 98.9% | 98.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2199
- ORF length:
- 2130
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagcgcaca gcaggcaaag agctggccct ggcaccgctg caggactggg 121 gtgaagagac cgaggacggc gcggtgtaca gtgtctccct gcggcggcag cgcagtcagc 181 gcaggagccc ggcggagggc cccgggggca gccaggctcc cagccccatt gccaatacct 241 tcctccacta tcgaaccagc aaggtgaggg tgctgagggc agcgcgcctg gagcggctgg 301 tgggagagtt ggtgtttgga gaccgtgagc aggaccccag cttcatgccc gccttcctgg 361 ccacctaccg gacctttgta cccactgcct gcctgctggg ctttctgctg ccaccaatgc 421 caccgccccc acctcccggg gtagagatca agaagacagc ggtacaagat ctgagcttca 481 acaagaacct gagggctgtg gtgtcagtgc tgggctcctg gctgcaggac caccctcagg 541 atttccgaga cccccctgtc cattcggacc tgggcagtgt ccgaaccttt ctgggctggg 601 cggccccagg gagtgctgag gctcaaaaag cagagaagct tctggaagat tttttggagg 661 aggctgagcg agagcaggaa gaggagccgc ctcaggtgtg gacaggacct cccagagttg 721 cccaaacttc tgacccagac tcttcagagg cctgcgcgga ggaagaggaa gggctcatgc 781 ctcaaggtcc ccagctcctg gacttcagcg tggacgaggt ggccgagcag ctgaccctca 841 tagacttgga gctcttctcc aaggtgaggc tctacgagtg cttgggctcc gtgtggtcgc 901 agagggaccg gccgggggct gcaggcgcct cccccactgt gcgcgccacc gtggcccagt 961 tcaacaccgt gaccggctgt gtgctgggtt ccgtgctcgg agcaccgggc ttggccgccc 1021 cgcagagggc gcagcggctg gagaagtgga tccgcatcgc ccagcgctgc cgagaactgc 1081 ggaacttctc ctccttgcgc gccatcctgt ccgccctgca atctaacccc atctaccggc 1141 tcaagcgcag ctggggggcc gtgagccggg aaccgctatc tactttcagg aaactttcgc 1201 agattttctc cgatgagaac aaccacctca gcagcagaga gattcttttc caggaggagg 1261 ccactgaggg atcccaagaa gaggacaaca ccccaggcag cctgccctca aaaccacccc 1321 caggccctgt cccctacctt ggcaccttcc ttacggacct ggttatgctg gacacagccc 1381 tgccggatat gttggagggg gatctcatta actttgagaa gaggaggaag gagtgggaga 1441 tcctggcccg catccagcag ctgcagaggc gctgtcagag ctacaccctg agcccccacc 1501 cgcccatcct ggctgccctg catgcccaga accagctcac cgaggagcag agctaccggc 1561 tctcccgggt cattgagcca ccagctgcct cctgccccag ctccccacgc atccgacggc 1621 ggatcagcct caccaagcgt ctcagtgcga agcttgcccg agagaaaagc tcatcaccta 1681 gtgggagtcc cggggatccc tcatccccca cctccagtgt gtccccaggg tcacccccct 1741 caagtcctag aagcagagat gctcctgctg gcagtccccc ggcctctcca gggccccagg 1801 gccccagcac caagctgccc ctgagcctgg acctgcccag cccccggccc ttcgctttgc 1861 ctctgggcag ccctcgaatc cccctcccgg cgcagcagag ctcggaggcc cgtgtcatcc 1921 gcgtcagcat cgacaatgac cacgggaacc tgtatcgaag catcttgctg accagtcagg 1981 acaaagcccc cagcgtggtc cggcgagcct tgcagaagca caatgtgccc cagccctggg 2041 cctgtgacta tcagctcttt caagtccttc ctggggaccg ggtgctcctg attcctgaca 2101 atgccaacgt cttctatgcc atgagtccag tcgcccccag agacttcatg ctgcggcgga 2161 aagaggggac ccggaacact ctgtctgtct ccccaagctt gccaactttc ttgtacaaag 2221 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 2281 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta