Construct: ORF ccsbBroad304_08715
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000603.1_s304c1, BRDN0000408322
- Derived from:
- ccsbBroadEn_08715
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SALL4 (57167)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08715
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57167 | SALL4 | spalt like transcription fa... | NM_020436.5 | 99.9% | 100% | 540T>C;1056G>A;2640G>C |
| 2 | human | 57167 | SALL4 | spalt like transcription fa... | XM_005260467.4 | 90.2% | 90.3% | (many diffs) |
| 3 | human | 57167 | SALL4 | spalt like transcription fa... | XM_011528921.2 | 90.2% | 90.3% | (many diffs) |
| 4 | human | 57167 | SALL4 | spalt like transcription fa... | XM_011528922.2 | 90.2% | 90.3% | (many diffs) |
| 5 | human | 57167 | SALL4 | spalt like transcription fa... | NM_001318031.2 | 58.4% | 58.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3228
- ORF length:
- 3159
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcgaggcgc aagcaggcga aaccccagca catcaactcg gaggaggacc 121 agggcgagca gcagccgcag cagcagaccc cggagtttgc agatgcggcc ccagcggcgc 181 ccgcggcggg ggagctgggt gctccagtga accacccagg gaatgacgag gtggcgagtg 241 aggatgaagc cacagtaaag cggcttcgtc gggaggagac gcacgtctgt gagaaatgct 301 gtgcggagtt cttcagcatc tctgagttcc tggaacataa gaaaaattgc actaaaaatc 361 cacctgtcct catcatgaat gacagcgagg ggcctgtgcc ttcagaagac ttctccggag 421 ctgtactgag ccaccagccc accagtcccg gcagtaagga ctgtcacagg gagaatggcg 481 gcagctcaga ggacatgaag gagaagccgg atgcggagtc tgtggtgtac ctaaagacag 541 agacagccct gccacccacc ccccaggaca taagctattt agccaaaggc aaagtggcca 601 acactaacgt gaccttgcag gcactacggg gcaccaaggt ggcggtgaat cagcggagcg 661 cggatgcact ccctgccccc gtgcctggtg ccaacagcat cccgtgggtc ctcgagcaga 721 tcttgtgtct gcagcagcag cagctacagc agatccagct caccgagcag atccgcatcc 781 aggtgaacat gtgggcctcc cacgccctcc actcaagcgg ggcaggggcc gacactctga 841 agaccttggg cagccacatg tctcagcagg tttctgcagc tgtggctttg ctcagccaga 901 aagctggaag ccaaggtctg tctctggatg ccttgaaaca agccaagcta cctcacgcca 961 acatcccttc tgccaccagc tccctgtccc cagggctggc acccttcact ctgaagccgg 1021 atgggacccg ggtgctcccg aacgtcatgt cccgcctccc gagcgctttg cttcctcagg 1081 ccccgggctc ggtgctcttc cagagccctt tctccactgt ggcactagac acatccaaga 1141 aagggaaggg gaagccaccg aacatctccg cggtggatgt caaacccaaa gacgaggcgg 1201 ccctctacaa gcacaagtgt aagtactgta gcaaggtttt tgggactgat agctccttgc 1261 agatccacct ccgctcccac actggagaga gacccttcgt gtgctctgtc tgtggtcatc 1321 gcttcaccac caagggcaac ctcaaggtgc actttcaccg acatccccag gtgaaggcaa 1381 acccccagct gtttgccgag ttccaggaca aagtggcggc cggcaatggc atcccctatg 1441 cactctctgt acctgacccc atagatgaac cgagtctttc tttagacagc aaacctgtcc 1501 ttgtaaccac ctctgtaggg ctacctcaga atctttcttc ggggactaat cccaaggacc 1561 tcacgggtgg ctccttgccc ggtgacctgc agcctgggcc ttctccagaa agtgagggtg 1621 gacccacact ccctggggtg ggaccaaact ataattcccc aagggctggt ggcttccaag 1681 ggagtgggac ccctgagcca gggtcagaga ccctgaaatt gcagcagttg gtggagaaca 1741 ttgacaaggc caccactgat cccaacgaat gtctcatttg ccaccgagtc ttaagctgtc 1801 agagctccct caagatgcat tatcgcaccc acaccgggga gagaccgttc cagtgtaaga 1861 tctgtggccg agccttttct accaaaggta acctgaagac acaccttggg gttcaccgaa 1921 ccaacacatc cattaagacg cagcattcgt gccccatctg ccagaagaag ttcactaatg 1981 ccgtgatgct gcagcaacat attcggatgc acatgggcgg tcagattccc aacacgcccc 2041 tgccagagaa tccctgtgac tttacgggtt ctgagccaat gaccgtgggt gagaacggca 2101 gcaccggcgc tatctgccat gatgatgtca tcgaaagcat cgatgtagag gaagtcagct 2161 cccaggaggc tcccagcagc tcctccaagg tccccacgcc tcttcccagc atccactcgg 2221 catcacccac gctagggttt gccatgatgg cttccttaga tgccccaggg aaagtgggtc 2281 ctgccccttt taacctgcag cgccagggca gcagagaaaa cggttccgtg gagagcgatg 2341 gcttgaccaa cgactcatcc tcgctgatgg gagaccagga gtatcagagc cgaagcccag 2401 atatcctgga aaccacatcc ttccaggcac tctccccggc caatagtcaa gccgaaagca 2461 tcaagtcaaa gtctcccgat gctgggagca aagcagagag ctccgagaac agccgcactg 2521 agatggaagg tcggagcagt ctcccttcca cgtttatccg agccccgccg acctatgtca 2581 aggttgaagt tcctggcaca tttgtgggac cctcgacatt gtccccaggg atgacccctt 2641 tgttagcagc ccagccacgc cgacaggcca agcaacatgg ctgcacacgg tgtgggaaga 2701 acttctcctc tgctagcgct cttcagatcc acgagcggac tcacactgga gagaagcctt 2761 ttgtgtgcaa catttgtggg cgagctttta ccaccaaagg caacttaaag gttcactaca 2821 tgacacacgg ggcgaacaat aactcagccc gccgtggaag gaagttggcc atcgagaaca 2881 ccatggctct gttaggtacg gacggaaaaa gagtctcaga aatctttccc aaggaaatcc 2941 tggccccttc agtgaatgtg gaccctgttg tgtggaacca gtacaccagc atgctcaatg 3001 gcggtctggc cgtgaagacc aatgagatct ctgtgatcca gagtgggggg gttcctaccc 3061 tcccggtttc cttgggggcc acctccgttg tgaataacgc cactgtctcc aagatggatg 3121 gctcccagtc gggtatcagt gcagatgtgg aaaaaccaag tgctactgac ggcgttccca 3181 aacaccagtt tcctcacttc ctggaagaaa acaagattgc ggtcagcttg ccaactttct 3241 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 3301 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta