Construct: ORF ccsbBroad304_08798
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013626.1_s304c1, BRDN0000408795
- Derived from:
- ccsbBroadEn_08798
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC81 (60494)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08798
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60494 | CCDC81 | coiled-coil domain containi... | NM_021827.4 | 99.9% | 100% | 987G>A |
| 2 | human | 60494 | CCDC81 | coiled-coil domain containi... | XM_017018105.2 | 88.2% | 83.8% | (many diffs) |
| 3 | human | 60494 | CCDC81 | coiled-coil domain containi... | NM_001156474.2 | 86.1% | 86.1% | 286_555del;1257G>A |
| 4 | human | 60494 | CCDC81 | coiled-coil domain containi... | XM_005274161.1 | 78.7% | 78.8% | 286_555del;966_967ins144;1113G>A |
| 5 | human | 60494 | CCDC81 | coiled-coil domain containi... | XM_005274162.4 | 64.5% | 64.5% | 0_1ins597;390G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1755
- ORF length:
- 1686
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gttggatacg atcgcccgtg ccctgcagga cctgggcagg caggtgctgc 121 ccactctgcc ctcgctgagc caggaggaag tctctattat ctgggggaat gtatcagaat 181 ttgtgagacg gcagttaacc ctgcacaagg gggttcagat tccagcattt ggaactttca 241 ctttcataag acaaaagctt gaggtgggaa acaacaaatt tatcttaatc cagaggcctg 301 tgtttatcat ggtggagaag ctagtgcaga ttcatggact caaacaaaac aaaaggcctg 361 gcactgtgga ctcggtgttg tctagcagag aggccttgag gaagtggccc agcagtgtgc 421 ttgcgtttcc aaggattgag ctcaaggaga tggaaaacaa actgcctatg gagacccttg 481 tagaagaatg tggagagaat agagaaagga agtgcaagtt aaaagaccag tcagacaaag 541 aagaaggcac cagagatatc tcatcaccca aaagacttcg agatagacaa gctttgttcc 601 ctgccaaagt gacaaatgtc agcttgctgg aaaagtttga acgaagtgag agtggtggga 661 agattatgac ccctgaaagc ttatcatatc caagttgtct gaaacacgac agtgagatga 721 agccccaaac atctccagct tgccaggatc ataacaaggc aggacaggaa atgtgctatg 781 tatgtttgca acgagcacaa cgaaattccc tgttgtacta cagtgaggaa aggaggagag 841 agatagaaga tgagagactc atacagcagt atcagatgtt aaaggatcag gaggctctct 901 tcagacacca gatgaaaagt ctggctacta gagaacagaa tcagaaaaat gctgcctata 961 atcttggagt tgctgaagct ataagaaacc acaagaatga gaaaccggaa ttttataaat 1021 ccttcctatt tgacaaacgg ccactcagtc ctgcacttaa tgctcttaag caagaggaat 1081 attcccggag tctcctgaaa caaatggata acagacagga aaacgaaata aagcaaagac 1141 aatacagaga gttgatggac cgcctggaac aagtgcaact cacagaggaa cttgctgcgc 1201 aaagagcgaa atttttaaaa gataagatgg aagaaacaca gtgttacaag agagctttgg 1261 atgcacagat aaagaacaaa ccctctcggc tgcccccctt tgagccagac tcctctgagc 1321 ccatctttgg taagaatgag ggtgaactga tggtggaaaa gcaaaagcga gaacaaaatt 1381 acatgaaaca ccagctggag gcagctgcta accacaagag gaaagccatc ctgcatcaac 1441 tagtggacca gaggcgggat ttgcaaatgc ttcagaggac acaaagagag cacttggcag 1501 acagaaccgc tgagctggag cgagtaaata gagtcaacca atgcttacag gaggactggg 1561 aaaggagtgc tgcgatgaag aagcagcgag acctggagga caaggctttt gaacgggctt 1621 cagacaagct gtttctccta gaccagtgtg agaagtatcg gcgctgcaag caatgccaga 1681 ggcgcacctc caacgtgggc gagagcaacc tgtggcccct gaacaagttc ctgcctggct 1741 cccggttgct tgtgttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 1801 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 1861 cgacaatcaa cctctggatt a