Construct: ORF ccsbBroad304_08824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015018.1_s304c1, BRDN0000407247
- Derived from:
- ccsbBroadEn_08824
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDH23 (64072)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08824
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64072 | CDH23 | cadherin related 23 | NM_001171931.2 | 99.9% | 100% | 366T>C;2316T>C;2388T>C |
| 2 | human | 64072 | CDH23 | cadherin related 23 | NM_001171930.2 | 76.2% | 74.5% | (many diffs) |
| 3 | human | 64072 | CDH23 | cadherin related 23 | NM_052836.4 | 48.8% | 45.6% | (many diffs) |
| 4 | human | 64072 | CDH23 | cadherin related 23 | NM_001171932.1 | 37.3% | 34.8% | (many diffs) |
| 5 | human | 64072 | CDH23 | cadherin related 23 | NM_022124.6 | 31.4% | 30.8% | (many diffs) |
| 6 | mouse | 22295 | Cdh23 | cadherin 23 (otocadherin) | XM_017313928.1 | 28.4% | 29.8% | (many diffs) |
| 7 | mouse | 22295 | Cdh23 | cadherin 23 (otocadherin) | XM_017313926.1 | 28.2% | 29.6% | (many diffs) |
| 8 | mouse | 22295 | Cdh23 | cadherin 23 (otocadherin) | XM_017313927.1 | 28.2% | 29.6% | (many diffs) |
| 9 | mouse | 22295 | Cdh23 | cadherin 23 (otocadherin) | NM_023370.3 | 27.9% | 29.3% | (many diffs) |
| 10 | mouse | 22295 | Cdh23 | cadherin 23 (otocadherin) | NM_001252635.1 | 27.9% | 29.2% | (many diffs) |
| 11 | mouse | 22295 | Cdh23 | cadherin 23 (otocadherin) | XM_017313930.1 | 27.8% | 29.2% | (many diffs) |
| 12 | mouse | 22295 | Cdh23 | cadherin 23 (otocadherin) | NR_045556.1 | 24.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3249
- ORF length:
- 3183
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaagag 61 ttggcatggg gcgccatgtt gccaccagct gccacgtggc ctggcttttg gtgctgatct 121 ctggatgctg gggccaggtg aaccggctgc ccttcttcac caaccacttc tttgatacat 181 acctgctgat cagcgaggac acgcctgtgg gttcttctgt gacccagttg ctggcccaag 241 acatggacaa tgaccccctg gtgtttggcg tgtctgggga ggaggcctct cgcttctttg 301 cagtggagcc tgacactggc gtggtgtggc tccggcagcc actggacaga gagaccaagt 361 cagagttcac cgtggagttc tctgtcagcg accaccaggg ggtgatcaca cggaaggtga 421 acatccaggt cggggatgtg aatgacaacg cgcccacatt tcacaatcag ccctacagcg 481 tccgcatccc tgagaataca ccagtgggga cgcccatctt catcgtgaat gccacagacc 541 ccgacttggg ggcagggggc agcgtcctct actccttcca gcccccctcc caattcttcg 601 ccattgacag cgcccgcggt atcgtcacag tgatccggga gctggactac gagaccacac 661 aggcctacca gctcacggtc aacgccacag atcaagacaa gaccaggcct ctgtccaccc 721 tggccaactt ggccatcatc atcacagatg tccaggacat ggaccccatc ttcatcaacc 781 tgccttacag caccaacatc tacgagcatt ctcctccggg cacgacggtg cgcatcatca 841 ccgccataga ccaggataaa ggacgtcccc ggggcattgg ctacaccatc gtttcaggga 901 ataccaacag catctttgcc ctggactaca tcagcggagt gctgaccttg aatggcctgc 961 tggaccggga gaaccccctg tacagccatg gcttcatcct gactgtgaag ggcacggagc 1021 tgaacgatga ccgcacccca tctgacgcta cagtcaccac gaccttcaat atcctggtta 1081 ttgacatcaa tgacaatgcc ccggagttca acagctccga gtacagcgtg gccatcactg 1141 agctggcaca ggtcggcttt gcccttccac tcttcatcca ggtggtggac aaggatgaga 1201 atttgggcct gaacagcatg tttgaggtgt acttggtggg gaacaactcc caccacttca 1261 tcatctcccc gacctccgtc caggggaagg cggacattcg tattcgggtg gccatcccac 1321 tggactacga gaccgtggac cgctacgact ttgatctctt tgccaatgag agtgtgcctg 1381 accatgtggg ctatgccaag gtgaagatca ctctcatcaa tgaaaatgac aaccggccca 1441 tcttcagcca gccactgtac aacatcagcc tgtacgagaa cgtcaccgtg gggacctctg 1501 tgctgacagt cctggcaact gacaatgatg caggcacctt tggggaagtc agctacttct 1561 tcagtgatga ccctgacagg ttctcgctgg acaaggacac gggactcatc atgctgattg 1621 ccaggctgga ctatgagctc atccagcgct tcaccctgac gatcattgcc cgggacgggg 1681 gcggcgagga gaccacaggc cgggtcagga tcaatgtgtt ggatgtcaac gacaacgtgc 1741 ccaccttcca gaaggatgcc tacgtgggtg ctctgcggga gaacgagcct tctgtcacac 1801 agctggtgcg gctccgggca acagatgaag actcccctcc caacaaccag atcacctaca 1861 gcattgtcag tgcatctgcc tttggcagct acttcgacat cagcctgtac gagggctatg 1921 gagtgatcag cgtcagtcgc cccctggatt atgaacagat atccaatggg ctgatttatc 1981 tgacggtcat ggccatggat gctggcaacc cccctctcaa cagcaccgtc cctgtcacca 2041 tcgaggtgtt tgatgagaat gacaaccctc ccaccttcag caagcccgcc tacttcgtct 2101 ccgtggtgga gaacatcatg gcaggagcca cggtgctgtt cctgaatgcc acagacctgg 2161 accgctcccg ggagtacggc caggagtcca tcatctactc cttggaaggc tccacccagt 2221 ttcggatcaa tgcccgctca ggggaaatca ccaccacgtc tctgcttgac cgagagacca 2281 agtctgaata catcctcatc gttcgcgcag tggacggggg tgtgggccac aaccagaaaa 2341 ctggcatcgc caccgtaaac atcaccctcc tggacatcaa cgacaaccac cccacgtgga 2401 aggacgcacc ctactacatc aacctggtgg agatgacccc tccagactct gacgtgacca 2461 cggtggtggc tgttgaccca gacctggggg agaatggcac cctggtgtac agcatccagc 2521 cacccaacaa gttctacagc ctcaacagca ccacgggcaa gatccgcacc acccacgcca 2581 tgctggaccg ggagaacccc gacccccatg aggccgagct gatgcgcaaa atcgtcgtct 2641 ctgttactga ctgtggcagg ccccctctga aagccaccag cagtgccaca gtgtttgtga 2701 acctcttgga tctcaatgac aatgacccca cctttcagaa cctgcctttt gtggccgagg 2761 tgcttgaagg catcccggcg ggggtctcca tctaccaagt ggtggccatc gacctcgatg 2821 agggcctgaa cggcctggtg tcctaccgca tgccggtggg catgccccgc atggacttcc 2881 tcatcaacag cagcagcggc gtggtggtca ccaccaccga gctggaccgc gagcgcatcg 2941 cggagtacca gctgcgggtg gtggccagtg atgcaggcac gcccaccaag agctccacca 3001 gcacgctcac catccatgtg ctggatgtga acgacgagac gcccaccttc ttcccggccg 3061 tgtacaatgt gtctgtgtcc gaggacgtgc cacgcgagtt ccgggtggtc tggctgaact 3121 gcacggacaa cgacgtgggc ctcaatgcag agctcagcta cttcatcaca ggtgctgccc 3181 cggcctccgc ccacctgtgc aggcctcctg gggccctgcc tccacccctc ccagatggac 3241 agccagacta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 3301 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 3361 tcaacctctg gatta