Construct: ORF ccsbBroad304_08839
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017227.1_s304c1, BRDN0000401811
- Derived from:
- ccsbBroadEn_08839
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFIH1 (64135)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08839
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64135 | IFIH1 | interferon induced with hel... | NM_022168.4 | 99.9% | 99.8% | 2528A>G;2836G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3144
- ORF length:
- 3075
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggctccat gtcgaatggg tattccacag acgagaattt ccgctatctc atctcgtgct 121 tcagggccag ggtgaaaatg tacatccagg tggagcctgt gctggactac ctgacctttc 181 tgcctgcaga ggtgaaggag cagattcaga ggacagtcgc cacctccggg aacatgcagg 241 cagttgaact gctgctgagc accttggaga agggagtctg gcaccttggt tggactcggg 301 aattcgtgga ggccctccgg agaaccggca gccctctggc cgcccgctac atgaaccctg 361 agctcacgga cttgccctct ccatcgtttg agaacgctca tgatgaatat ctccaactgc 421 tgaacctcct tcagcccact ctggtggaca agcttctagt tagagacgtc ttggataagt 481 gcatggagga ggaactgttg acaattgaag acagaaaccg gattgctgct gcagaaaaca 541 atggaaatga atcaggtgta agagagctac taaaaaggat tgtgcagaaa gaaaactggt 601 tctctgcatt tctgaatgtt cttcgtcaaa caggaaacaa tgaacttgtc caagagttaa 661 caggctctga ttgctcagaa agcaatgcag agattgagaa tttatcacaa gttgatggtc 721 ctcaagtgga agagcaactt ctttcaacca cagttcagcc aaatctggag aaggaggtct 781 ggggcatgga gaataactca tcagaatcat cttttgcaga ttcttctgta gtttcagaat 841 cagacacaag tttggcagaa ggaagtgtca gctgcttaga tgaaagtctt ggacataaca 901 gcaacatggg cagtgattca ggcaccatgg gaagtgattc agatgaagag aatgtggcag 961 caagagcatc cccggagcca gaactccagc tcaggcctta ccaaatggaa gttgcccagc 1021 cagccttgga agggaagaat atcatcatct gcctccctac agggagtgga aaaaccagag 1081 tggctgttta cattgccaag gatcacttag acaagaagaa aaaagcatct gagcctggaa 1141 aagttatagt tcttgtcaat aaggtactgc tagttgaaca gctcttccgc aaggagttcc 1201 aaccattttt gaagaaatgg tatcgtgtta ttggattaag tggtgatacc caactgaaaa 1261 tatcatttcc agaagttgtc aagtcctgtg atattattat cagtacagct caaatccttg 1321 aaaactccct cttaaacttg gaaaatggag aagatgctgg tgttcaattg tcagactttt 1381 ccctcattat cattgatgaa tgtcatcaca ccaacaaaga agcagtgtat aataacatca 1441 tgaggcatta tttgatgcag aagttgaaaa acaatagact caagaaagaa aacaaaccag 1501 tgattcccct tcctcagata ctgggactaa cagcttcacc tggtgttgga ggggccacga 1561 agcaagccaa agctgaagaa cacattttaa aactatgtgc caatcttgat gcatttacta 1621 ttaaaactgt taaagaaaac cttgatcaac tgaaaaacca aatacaggag ccatgcaaga 1681 agtttgccat tgcagatgca accagagaag atccatttaa agagaaactt ctagaaataa 1741 tgacaaggat tcaaacttat tgtcaaatga gtccaatgtc agattttgga actcaaccct 1801 atgaacaatg ggccattcaa atggaaaaaa aagctgcaaa agaaggaaat cgcaaagaac 1861 gtgtttgtgc agaacatttg aggaagtaca atgaggccct acaaattaat gacacaattc 1921 gaatgataga tgcgtatact catcttgaaa ctttctataa tgaagagaaa gataagaagt 1981 ttgcagtcat agaagatgat agtgatgagg gtggtgatga tgagtattgt gatggtgatg 2041 aagatgagga tgatttaaag aaacctttga aactggatga aacagataga tttctcatga 2101 ctttattttt tgaaaacaat aaaatgttga aaaggctggc tgaaaaccca gaatatgaaa 2161 atgaaaagct gaccaaatta agaaatacca taatggagca atatactagg actgaggaat 2221 cagcacgagg aataatcttt acaaaaacac gacagagtgc atatgcgctt tcccagtgga 2281 ttactgaaaa tgaaaaattt gctgaagtag gagtcaaagc ccaccatctg attggagctg 2341 gacacagcag tgagttcaaa cccatgacac agaatgaaca aaaagaagtc attagtaaat 2401 ttcgcactgg aaaaataaat ctgcttatcg ctaccacagt ggcagaagaa ggtctggata 2461 ttaaagaatg taacattgtt atccgttatg gtctcgtcac caatgaaata gccatggtcc 2521 aggcccgtgg tcgagccaga gctgatgaga gcacctacgt cctggttgct cacagtggtt 2581 caggagttat cgaacgtgag acagttaatg atttccgaga gaagatgatg tataaagcta 2641 tacattgtgt tcaaaatatg aaaccagagg agtatgctca taagattttg gaattacaga 2701 tgcaaagtat aatggaaaag aaaatgaaaa ccaagagaaa tattgccaag cattacaaga 2761 ataacccatc actaataact ttcctttgca aaaactgcag tgtgctagcc tgttctgggg 2821 aagatatcca tgtaattgag aaaatgcatc acgtcaatat gaccccagaa ttcaaggaac 2881 tttacattgt aagagaaaac aaaacactgc aaaagaagtg tgccgactat caaataaatg 2941 gtgaaatcat ctgcaaatgt ggccaggctt ggggaacaat gatggtgcac aaaggcttag 3001 atttgccttg tctcaaaata aggaattttg tagtggtttt caaaaataat tcaacaaaga 3061 aacaatacaa aaagtgggta gaattaccta tcacatttcc caatcttgac tattcagaat 3121 gctgtttatt tagtgatgag gatttgccca ctttcttgta caaagtggtt ggtaagccta 3181 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 3241 gcgttaagtc gacaatcaac ctctggatta