Construct: ORF ccsbBroad304_08851

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009326.1_s304c1, BRDN0000410139
Derived from:
ccsbBroadEn_08851
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LMBR1 (64327)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08851
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64327 LMBR1 limb development membrane p... NM_022458.4 99.9% 99.7% 1453G>A
2 human 64327 LMBR1 limb development membrane p... NM_001350953.2 92.2% 91.9% 758_880del;1576G>A
3 human 64327 LMBR1 limb development membrane p... NM_001363410.2 88.9% 88.7% 1224_1225ins162;1291G>A
4 human 64327 LMBR1 limb development membrane p... NM_001363412.2 83.3% 76.1% (many diffs)
5 human 64327 LMBR1 limb development membrane p... NM_001363409.2 82% 81.7% 758_880del;1347_1348ins162;1414G>A
6 human 64327 LMBR1 limb development membrane p... NM_001363411.2 74.8% 74.6% 0_1ins369;1084G>A
7 human 64327 LMBR1 limb development membrane p... NM_001350954.2 69.9% 66.5% (many diffs)
8 human 64327 LMBR1 limb development membrane p... NM_001350955.2 68.9% 68.7% 0_1ins456;997G>A
9 human 64327 LMBR1 limb development membrane p... NM_001350956.2 68.9% 68.7% 0_1ins456;997G>A
10 human 64327 LMBR1 limb development membrane p... NM_001363413.2 68.9% 68.7% 0_1ins456;997G>A
11 human 64327 LMBR1 limb development membrane p... XM_017012515.2 63.9% 54% (many diffs)
12 human 64327 LMBR1 limb development membrane p... NM_001350957.2 63.8% 63.6% 0_1ins369;855_856ins162;922G>A
13 human 64327 LMBR1 limb development membrane p... XM_005249558.2 61.7% 58.7% (many diffs)
14 human 64327 LMBR1 limb development membrane p... NM_001350958.2 57.8% 57.7% 0_1ins456;768_769ins162;835G>A
15 human 64327 LMBR1 limb development membrane p... NR_146959.1 30.5% 1_215del;1668G>A;1686_4814del
16 human 64327 LMBR1 limb development membrane p... XR_001744850.1 24.6% (many diffs)
17 human 64327 LMBR1 limb development membrane p... XR_428185.1 23.6% (many diffs)
18 human 64327 LMBR1 limb development membrane p... XR_002956477.1 18.2% 1_190del;1643G>A;1661_8052del
19 human 64327 LMBR1 limb development membrane p... XR_001744848.1 18.1% (many diffs)
20 human 64327 LMBR1 limb development membrane p... XR_002956481.1 18% (many diffs)
21 human 64327 LMBR1 limb development membrane p... XR_001744847.1 17.9% (many diffs)
22 human 64327 LMBR1 limb development membrane p... XR_002956482.1 16.5% (many diffs)
23 human 64327 LMBR1 limb development membrane p... NR_146958.2 16.5% (many diffs)
24 human 64327 LMBR1 limb development membrane p... XR_002956479.1 16.2% (many diffs)
25 human 64327 LMBR1 limb development membrane p... XR_002956480.1 16.2% (many diffs)
26 human 64327 LMBR1 limb development membrane p... XR_002956478.1 15.9% (many diffs)
27 mouse 56873 Lmbr1 limb region 1 NM_020295.3 89.6% 95.9% (many diffs)
28 mouse 56873 Lmbr1 limb region 1 XM_006535764.3 88.5% 94.4% (many diffs)
29 mouse 56873 Lmbr1 limb region 1 XM_017321034.1 84.7% 90.8% (many diffs)
30 mouse 56873 Lmbr1 limb region 1 XM_006535765.3 82.7% 85.3% (many diffs)
31 mouse 56873 Lmbr1 limb region 1 XM_011249782.2 82.7% 85.3% (many diffs)
32 mouse 56873 Lmbr1 limb region 1 XM_011249783.1 72.5% 75.5% (many diffs)
33 mouse 56873 Lmbr1 limb region 1 XM_006535767.3 67.5% 72.6% (many diffs)
34 mouse 56873 Lmbr1 limb region 1 XM_006535768.3 66.6% 71.8% (many diffs)
35 mouse 56873 Lmbr1 limb region 1 XM_006535770.3 63.8% 68.3% (many diffs)
36 mouse 56873 Lmbr1 limb region 1 XM_006535771.3 46.7% 49.3% (many diffs)
37 mouse 56873 Lmbr1 limb region 1 XM_006535772.3 46.4% 49.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1536
ORF length:
1470
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga agggcaggac gaggtgtcgg cgcgggagca gcacttccac agccaagtgc
121gggagtccac gatatgtttc cttctttttg ccattctcta cgttgtttcc tacttcatca
181tcacaagata caagagaaaa tcagatgaac aagaagatga agatgccatc gtcaacagga
241tttcgttgtt tttgagcacg ttcactctcg cagtgtcagc tggggctgtt ttgcttttac
301ccttctcaat catcagcaat gaaatcctgc tttcttttcc tcagaactac tatattcagt
361ggctaaatgg ctccctgatt catggtttgt ggaatcttgc ttcccttttt tccaaccttt
421gtttatttgt attgatgccc tttgcctttt tctttctgga atcagaaggc tttgctggcc
481tgaaaaaggg aatccgagcc cgcattttag agactttggt catgcttctt cttcttgcgt
541tactcattct tgggatagtg tgggtagctt cagcactcat tgacaacgat gccgcaagca
601tggaatcttt atatgatctc tgggagttct atctacccta tttatattcc tgtatatcat
661tgatgggatg tttgttactt ctcttgtgta caccagttgg cctttctcgt atgttcacag
721tgatgggtca gttgctagtg aagccaacaa ttcttgaaga cctggatgaa caaatttata
781tcattacctt agaggaagaa gcactccaga gacgactaaa tgggctgtct tcatcggtgg
841aatacaacat aatggagttg gaacaagaac ttgaaaatgt aaagactctt aagacaaaat
901tagagaggcg aaaaaaggct tcagcatggg aaagaaattt ggtgtatccc gctgttatgg
961ttctccttct tattgagaca tccatctcgg tcctcttggt ggcttgtaat attctttgcc
1021tattggttga tgaaacagca atgccaaaag gaacaagggg gcctggaata ggaaatgcct
1081ctctttctac gtttggtttt gtgggagctg cgcttgaaat cattttgatt ttctatctta
1141tggtgtcctc tgttgtcggc ttctatagcc ttcgattttt tggaaacttt actcccaaga
1201aagatgacac aactatgaca aagatcattg gaaattgtgt gtccatcttg gttttgagct
1261ctgctctgcc tgtgatgtcg agaacactgg gaatcactag atttgatcta cttggcgact
1321ttggaaggtt taattggctg ggaaatttct atattgtatt atcctacaat ttgctttttg
1381ctattgtgac aacattgtgt ctggtccgaa aattcacctc tgcagttcga gaagaacttt
1441tcaaggccct agggcttcat aaacttcact taccaaatac ttcaagggat tcagaaacag
1501ccaagccttc tgtaaatagg catcagaaag cactgtgccc aactttcttg tacaaagtgg
1561ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1621ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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