Construct: ORF ccsbBroad304_08960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012111.1_s304c1, BRDN0000462376
- Derived from:
- ccsbBroadEn_08960
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLMN (79789)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08960
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79789 | CLMN | calmin | NM_024734.4 | 99.9% | 99.8% | 391A>G;1847A>T |
| 2 | human | 79789 | CLMN | calmin | XM_011537158.1 | 96.9% | 96.8% | (many diffs) |
| 3 | human | 79789 | CLMN | calmin | XM_017021646.1 | 94.7% | 94.3% | (many diffs) |
| 4 | human | 79789 | CLMN | calmin | XM_017021647.1 | 92.2% | 92.2% | (many diffs) |
| 5 | human | 79789 | CLMN | calmin | XM_011537159.2 | 92.2% | 92% | (many diffs) |
| 6 | human | 79789 | CLMN | calmin | XR_429332.2 | 23% | (many diffs) | |
| 7 | human | 79789 | CLMN | calmin | XR_245721.2 | 23% | (many diffs) | |
| 8 | human | 79789 | CLMN | calmin | XR_001750558.1 | 22.9% | (many diffs) | |
| 9 | human | 79789 | CLMN | calmin | XR_429330.2 | 22.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3075
- ORF length:
- 3006
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgcacac gagtgggact ggttccaacg cgaggagctc atcgggcaga 121 ttagcgacat ccgagtgcag aacctgcaag ttgagaggga aaatgtgcag aagaggacct 181 ttacacgatg gataaatcta catctagaaa agtgcaaccc acctctagaa gttaaagatt 241 tattcgtcga tatacaagat ggcaaaatcc taatggcttt gttagaagtc ctgtctgggc 301 ggaatctgct gcacgaatac aaatcctcgt cgcatcgtat ttttcggttg aacaacatag 361 cgaaagcact taagtttttg gaagatagca atgtaaaact ggttagcatt gatgcagcag 421 aaatagcaga tggcaaccct tctttggttc ttgggctggt atggaacata atcctcttct 481 tccagattaa ggagctcaca ggcaacctca gcagaaactc tccatcttcc agcttgtccc 541 ctggctcagg gggcacagac tcagactcat ccttcccacc cacacccact gcagagagga 601 gcgtggcaat atcggtgaaa gaccagagga aggctatcaa ggccctgttg gcgtgggtgc 661 agaggaaaac gagaaagtat ggcgtggcgg tgcaggactt tgcgggcagt tggaggagtg 721 ggctggcttt cctggcggtg atcaaggcca ttgaccccag cctggtggac atgaaacagg 781 ccctggaaaa ttccacacga gaaaatctag agaaggcttt cagcatcgca caggatgccc 841 tgcacatccc caggctcctg gagccagaag acatcatggt tgacacacca gacgagcagt 901 ctatcatgac ttacgtggca cagtttctag aacgttttcc ggagttggaa gccgaagata 961 ttttcgattc agataaagaa gttcctatcg aatccacttt tgttcgcatc aaagaaactc 1021 cttctgaaca ggagagcaaa gtcttcgttc tgactgaaaa tggggagcgt acctacactg 1081 ttaaccatga aaccagccac ccaccaccct ccaaagtctt tgtctgtgac aagcccgaga 1141 gcatgaagga attccgcctg gatggtgttt ccagccatgc gctgtcagac agctccaccg 1201 agttcatgca ccagattatt gaccaggtcc tgcaaggggg cccaggtaag accagcgaca 1261 tcagtgagcc atctccagaa tcctccattt tatcatccag aaaggagaac gggaggtcca 1321 actctttgcc gatcaagaaa acagttcact ttgaggctga cacctacaag gatcctttct 1381 gcagtaagaa cctgtccctt tgctttgaag ggagcccaag agtggcaaag gaatcattga 1441 ggcaggatgg acatgtcttg gcagttgagg ttgctgagga aaaggaacag aaacaggaat 1501 cctcgaagat tccagaatcc tcctctgaca aggtcgctgg tgacattttt ttggtggagg 1561 gcacaaacaa taattctcag tcttcttcct gtaatggtgc tttagagagt acagcccgcc 1621 acgatgaaga aagtcactct ctttcacccc caggagaaaa tactgtgatg gccgattcct 1681 tccagatcaa ggttaacctg atgactgtag aagctttaga ggagggagac tattttgaag 1741 ccatcccatt aaaagcctca aaatttaaca gcgacctaat agattttgct tctaccagcc 1801 aggctttcaa caaagttcct tcacctcatg agacaaaacc tgacgaggat gctgaggctt 1861 ttgagaatca tgctgaaaaa ctaggtaaaa ggagtattaa atctgctcac aaaatgaagg 1921 attcgccaga gcctcaagtt aagatggaca aacatgaacc tcatcaggac tccggagaag 1981 aagctgaagg ctgtccttca gccccagaag agacaccagt ggataaaaag ccagaggtgc 2041 atgaaaaggc caagagaaag tccacccgtc ctcattatga ggaagaggga gaagacgatg 2101 acctccaggg tgtgggcgag gaattatctt ccagcccccc aagcagctgt gtcagcttgg 2161 agacccttgg gagtcacagc gaagaaggcc tggatttcaa gccctcccca cccctctcaa 2221 aggtttccgt cattccccac gacctcttct atttcccaca ctatgaggtt cccctggctg 2281 cagttttgga ggcttatgta gaagacccgg aggatctaaa aaatgaagaa atggatctcg 2341 aagagccaga gggctatatg ccagacctgg actccaggga ggaggaggcc gatggctctc 2401 agagcagctc cagttcctcg gtgccaggag agagcctccc cagtgccagc gaccaggtgc 2461 tgtatctcag caggggtggt gtgggtacca caccagcctc agaacccgct ccactggccc 2521 cccatgagga ccaccagcaa agggagacca aagagaatga ccccatggac agccatcagt 2581 cccaggaatc cccaaacctg gaaaacatag caaaccccct agaagaaaat gtaacgaaag 2641 aatcaatcag tagtaaaaaa aaggaaaaaa ggaaacatgt ggaccacgta gaaagttcac 2701 tatttgtagc accaggaagt gttcaatcct cagatgacct agaagaagac agtagcgact 2761 acagcattcc ttccaggact agtcacagtg actccagcat ttaccttcga cgacatactc 2821 ataggtcttc ggaatcggat cattttagct atgttcagtt gaggaacgca gcagatctgg 2881 atgacagaag aaaccgaata ttaaccagga aggccaacag ctcaggagaa gccatgtcac 2941 tggggagcca cagcccgcag agtgactccc tgacacagct tgtccagcag ccggatatga 3001 tgtattttat tctcttcctg tggctcctgg tgtactgctt gctgctcttc ccacaactgg 3061 atgttagcag gctcttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 3121 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 3181 cgacaatcaa cctctggatt a