Construct: ORF ccsbBroad304_08979

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013572.1_s304c1, BRDN0000400447
Derived from:
ccsbBroadEn_08979
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPP21 (79897)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_08979
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79897 RPP21 ribonuclease P/MRP subunit p21 NM_024839.3 99.7% 99.3% 231G>C
2 human 79897 RPP21 ribonuclease P/MRP subunit p21 NM_001199120.2 94.8% 94.4% 158_181del;255G>C
3 human 79897 RPP21 ribonuclease P/MRP subunit p21 NM_001199121.2 90.4% 75.1% 231G>C;366_372delAGGTGAG;432_433ins37
4 human 202658 TRIM39-RPP21 TRIM39-RPP21 readthrough NM_001199119.1 29.5% 26.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
528
ORF length:
462
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggggccggtg aaggaccgcg aggccttcca gaggctcaac ttcctgtacc
121aggccgccca ttgtgtcctt gcccaggacc ccgagaacca ggcgctggcg aggttttact
181gctacactga gaggaccatt gcgaagcggc tcgtcttgcg gcgggatccc tcggtgaaga
241ggactctctg tcgaggctgc tcttccctcc tcgtcccggg cctcacctgc acccaccgcc
301agagacgctg caggggacag cgctggaccg tacagacctg cctaacatgc cagcgcagcc
361aacgcttcct caatgatccc gggcatttac tctggggaga caggcctgag gcccagctcg
421ggagccaagc agattccaaa ccactacaac ccttgccaaa cacagcccac tccatttcag
481accgccttcc tgaggagaaa atgcagactc agggttccag taaccagtgc ccaactttct
541tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
601aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)