Construct: ORF ccsbBroad304_09006
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002300.1_s304c1, BRDN0000399670
- Derived from:
- ccsbBroadEn_09006
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPF1 (80135)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09006
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80135 | RPF1 | ribosome production factor ... | NM_025065.7 | 99.9% | 99.7% | 25A>G |
| 2 | mouse | 70285 | Rpf1 | ribosome production factor ... | NM_027371.3 | 88% | 95.1% | (many diffs) |
| 3 | mouse | 70285 | Rpf1 | ribosome production factor ... | XM_006502050.3 | 84.2% | 90.4% | (many diffs) |
| 4 | mouse | 70285 | Rpf1 | ribosome production factor ... | XM_006502051.3 | 53.7% | 54.2% | (many diffs) |
| 5 | mouse | 70285 | Rpf1 | ribosome production factor ... | NM_027332.3 | 51.4% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gaaagccggg gataagagcg gcagcagcgg gaagaaaagt ctaaaacgga 121 aagccgctgc cgaagaactt caggaggctg caggcgctgg ggatggggcg acggaaaacg 181 gggtccaacc cccgaaagcg gctgcctttc cgccaggctt tagcatttcg gagattaaaa 241 acaaacagcg gcgacactta atgttcacgc ggtggaaaca gcagcagcgg aaggaaaagt 301 tggcagctaa gaaaaaactt aaaaaagaaa gagaggctct tggcgataag gctccaccaa 361 agcctgtacc caagaccatt gacaaccagc gagtgtatga tgaaaccaca gtagacccta 421 atgatgaaga ggtcgcttat gatgaagcta cagatgaatt tgcttcttac ttcaacaaac 481 agacttctcc caagattctc atcacaacat cagatagacc tcatgggaga acagtacgac 541 tctgtgaaca gctctccaca gttataccaa actcacatgt ttattacaga agaggactgg 601 ctctgaaaaa aattattcca cagtgcatcg caagagattt cacagacctg attgttatta 661 atgaagatcg taaaacccca aatggactta ttttgagtca cttgccaaat ggcccaactg 721 ctcattttaa aatgagcagt gttcgtcttc gtaaagaaat taagagaaga ggcaaggacc 781 ccacagaaca catacctgaa ataattctga ataattttac aacacggctg ggtcattcaa 841 ttggacgtat gtttgcatct ctctttcctc ataatcctca atttatcgga aggcaggttg 901 ccacattcca caatcaacgg gattacatat tcttcagatt tcacagatac atattcagga 961 gtgaaaagaa agtgggaatt caggaacttg gaccacgttt taccttaaaa ttaaggtctc 1021 ttcagaaagg aacctttgat tctaaatatg gagagtatga atgggtccat aagccccggg 1081 aaatggatac aagtagaaga aaattccatt tatacccaac tttcttgtac aaagtggttg 1141 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1201 accggtggcg cgttaagtcg acaatcaacc tctggatta