Construct: ORF ccsbBroad304_09097
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007745.1_s304c1, BRDN0000460544
- Derived from:
- ccsbBroadEn_09097
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB33B (83452)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09097
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83452 | RAB33B | RAB33B, member RAS oncogene... | NM_031296.3 | 99.8% | 99.5% | 182G>A |
| 2 | human | 83452 | RAB33B | RAB33B, member RAS oncogene... | XM_011532299.1 | 82.5% | 82.3% | 1_144del;326G>A |
| 3 | mouse | 19338 | Rab33b | RAB33B, member RAS oncogene... | NM_016858.2 | 87.5% | 94.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 756
- ORF length:
- 687
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgaggag atggagtcgt cgctcgaggc aagcttttcg tccagcgggg 121 cagtgtcagg ggcctcaggg tttttgcctc ctgcccgctc ccgcatcttc aagataatcg 181 tgatcggcga ctccaatgtg ggcaagacat gcctgaccta ccgcttctgc gctggccgct 241 tccccgacca caccgaggcc acgatagggg tggatttccg agaacgagcg gtggagattg 301 atggggagcg catcaagatc cagctatggg acacagcagg acaagaacga ttcagaaaga 361 gcatggttca gcactactac agaaatgtac atgctgttgt cttcgtgtat gatatgacca 421 acatggctag ttttcatagc ctaccatctt ggatagaaga atgcaaacaa catttgctag 481 ccaatgatat accacggatt cttgttggaa ataaatgtga cttgagaagt gccatacagg 541 tacccacaga cttggcacaa aaatttgctg acacacacag tatgcctttg tttgaaacgt 601 ctgctaaaaa ccccaatgat aatgaccatg tggaagctat atttatgacc ttggctcata 661 agcttaagag ccacaaacca ttaatgctta gtcagccccc tgataatgga attatcctga 721 agcctgaacc aaagcctgca atgacgtgct ggtgcttgcc aactttcttg tacaaagtgg 781 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 841 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta