Construct: ORF ccsbBroad304_09116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002485.1_s304c1, BRDN0000401819
- Derived from:
- ccsbBroadEn_09116
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP26 (83844)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09116
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83844 | USP26 | ubiquitin specific peptidas... | NM_031907.2 | 99.9% | 99.8% | 576G>A;2101T>A |
| 2 | human | 83844 | USP26 | ubiquitin specific peptidas... | XM_017029892.1 | 99.9% | 99.8% | 576G>A;2101T>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2808
- ORF length:
- 2739
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgcccta ttcctacgtg gttttgtcca aatagggaac tgcaagactg 121 ggatatctaa gtcaaaagaa gcattcattg aagcagtgga aagaaagaag aaagatagac 181 tggtgctgta tttcaaaagt ggaaaatata gcacttttcg gctaagtgat aatattcaaa 241 atgtagtcct taaatcctat agaggaaacc aaaatcacct gcatttaact ttacaaaata 301 ataatggctt gtttattgaa ggattatcct ccacagatgc tgaacaattg aagatattct 361 tggacagagt tcatcaaaac gaggttcagc cacctgtgag acctggtaag ggtgggagtg 421 tcttttctag cacaacacag aaggaaatca acaaaacttc attccacaaa gttgatgaga 481 aatcaagtag caaatctttt gagatagcaa aaggaagtgg gacaggtgtc cttcagagga 541 tgcctttgct tacatcaaaa ttgacactta cttgcggaga gttatcagaa aatcagcaca 601 agaagaggaa aagaatgctc tcatctagct cagagatgaa tgaagaattc ttgaaagaaa 661 ataattctgt agaatacaag aaatccaagg cagattgttc gaggtgtgta agctataatc 721 gagagaaaca attgaagtta aaagagttag aagagaataa gaaattggaa tgtgaatctt 781 catgcatcat gaacgccact ggaaatcctt acctagatga cattggtctt ctccaagctc 841 tcactgagaa aatggttttg gtatttctgt tacaacaagg gtatagtgac ggttacacaa 901 agtgggataa attaaaacta ttttttgaat tatttccaga gaaaatatgc cacggcctcc 961 ccaatttggg aaacacctgt tatatgaatg cagtgttaca gtctctactt tcaatcccat 1021 cgtttgctga tgatttactt aatcagagtt tcccatgggg taaaattccc cttaatgctc 1081 ttaccatgtg cttggcacgg ctactttttt ttaaagatac ctataatata gaaatcaagg 1141 agatgttact cttgaatctt aaaaaggcca tttcagcagc tgcagagata ttccatggca 1201 atgcacagaa cgatgctcat gagtttttag ctcactgttt agatcaactg aaagataaca 1261 tggaaaaact caacacaatt tggaagccta aaagtgaatt tggggaagat aattttccta 1321 aacaggtttt tgctgatgat cctgacacca gtgggttttc ttgccctgtc attactaatt 1381 ttgagttaga gttgttgcac tccattgctt gtaaagcttg tggtcaggtt attctcaaga 1441 cagaactgaa taattacctc tccatcaacc ttccccaaag aataaaagca catccttcat 1501 ctattcagtc tacttttgat cttttttttg gagcagaaga gcttgagtat aaatgtgcaa 1561 aatgtgagca caagacttcc gttggagtgc actcattcag taggctacct agaatcctta 1621 ttgttcacct caaacgctat agcttgaatg agttttgtgc attaaagaag aatgaccagg 1681 aagtcatcat ttccaaatat ttaaaggtgt cttctcattg caatgaaggc accagaccac 1741 ctcttccctt gagtgaggat ggagaaatta cagatttcca attattaaaa gttattcgaa 1801 agatgacttc tggaaacatc agtgtatcat ggcctgcaac aaaggaatcc aaagatatcc 1861 tggctccaca cattggatca gataaggagt ctgaacaaaa aaaaggccag acagtcttta 1921 aaggggcaag cagaagacag cagcaaaagt accttggaaa aaattctaaa ccaaatgagc 1981 tagaatctgt atactcagga gatcgagcat tcattgaaaa agaaccgtta gctcacttaa 2041 tgacgtatct ggaagatacc tcactttgtc agttccacaa agctggaggt aaacctgcca 2101 gcagcccagg cacacctctc tcaaaagttg actttcaaac agtgcccgaa aatccaaaac 2161 gaaagaaaaa tgtgaaaacc agtaagtttg tagcttttga taggattatc aatcctacta 2221 aagatttgta tgaagataaa aatatcagaa ttccagaaag attccaaaaa gtgtctgaac 2281 agactcagca gtgtgacggt atgagaatct gtgaacaagc ccctcagcag gcactgcctc 2341 aaagctttcc aaagccaggc acccaggggc acacaaagaa cctcctaaga cctacaaaat 2401 taaatctaca gaagtctaac aggaattccc tacttgcact gggttccaat aagaatccaa 2461 gaaacaaaga cattttagat aagataaaat ctaaagccaa ggaaacaaaa agaaatgatg 2521 ataagggaga tcatacctac cggctcatta gtgttgtcag ccatcttggg aagactctaa 2581 agtcaggcca ttatatctgt gatgcctatg actttgagaa acagatctgg ttcacttacg 2641 atgatatgcg ggtgttaggt atccaggagg cccagatgca ggaggatagg cgttgcactg 2701 ggtacatctt cttttacatg cataatgaga tctttgaaga gatgttgaaa agagaagaga 2761 atgcccagct taatagcaag gaggtagagg agacccttca gaaggaattg ccaactttct 2821 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2881 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta