Construct: ORF ccsbBroad304_09149
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007649.1_s304c1, BRDN0000461209
- Derived from:
- ccsbBroadEn_09149
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C4orf17 (84103)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09149
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84103 | C4orf17 | chromosome 4 open reading f... | NM_032149.3 | 99.7% | 99.1% | 191G>A;253T>C;271G>A |
| 2 | human | 84103 | C4orf17 | chromosome 4 open reading f... | XM_011532315.2 | 52.3% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1146
- ORF length:
- 1077
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacctcaac cccccgacat ctgctcttca gatcgagggc aaaggcagcc 121 atattatggc tagaaatgta agctgctttc tagtcaggca cacccctcat cccagaagag 181 tctgccacat caaaggcttg aataacattc caatctgtac tgtgaatgat gatgagaatg 241 catttggaac attgtgggaa gttggccagt ctaactactt agagaagaac aggataccat 301 ttgccaattg cagttacccc cccagcactg cagtccagaa gagccctgta agaggaatgt 361 cgccagcccc aaacggtgcc aaagtgcctc cacggcctca ttctgagccc agtagaaaaa 421 ttaaagagtg cttcaaaact tccagtgaga atcccttagt aattaaaaag gaagaaatta 481 aggccaaaag accaccatca cctccaaagg catgctctac tcctggctcc tgttcttcag 541 ggatgacaag taccaagaat gatgtgaaag caaacaccat ttgcatacca aactatctgg 601 atcaggaaat aaaaatcctg gcaaagctct gtagcatttt gcatactgat tctctggcag 661 aagttttaca gtggctgctt catgcaactt caaaagaaaa agagtgggtc tcagctttga 721 ttcattctga gcttgccgag ataaacctgt taactcatca cagaagaaac acctcaatgg 781 aaccagcagc agagactggg aagccaccca cagttaaatc accacccaca gttaaattgc 841 ccccaaattt tactgcaaaa tcaaaagtgc tgaccagaga tacagaaggg gatcaaccaa 901 ccagagtgtc aagtcaagga tctgaagaaa acaaggaagt accaaaagag gctgagcaca 961 agcctccact acttataaga agaaataata tgaaaatacc tgttgcagaa tatttcagca 1021 aaccaaattc tcctcccagg cctaacactc aggagagtgg atcagcaaaa ccagtgtcag 1081 caaggagtat acaagaatac aacctctgtc cccaaagagc atgttatcct tcaacacacc 1141 ggaggttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1201 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1261 acctctggat ta