Construct: ORF ccsbBroad304_09182

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005155.1_s304c1, BRDN0000410044
Derived from:
ccsbBroadEn_09182
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
THOC3 (84321)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09182
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84321 THOC3 THO complex 3 NM_032361.3 99.9% 100% 357C>T
2 human 84321 THOC3 THO complex 3 XM_017009985.1 74.7% 74.6% 1_1delAins265;93C>T
3 human 728554 LOC728554 THO complex 3 pseudogene NR_003615.2 57.3% (many diffs)
4 mouse 73666 Thoc3 THO complex 3 NM_028597.3 89.1% 98.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1119
ORF length:
1053
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggtccccgct gcagccatgg ggccctcggc gttgggccag agcggccccg
121gctcgatggc cccgtggtgc tcagtgagca gcggcccgtc gcgctacgtg cttgggatgc
181aggagctgtt ccggggccac agcaagacgc gcgagttcct ggcgcacagc gccaaggtgc
241actcggtggc ctggagttgc gacgggcgtc gcctagcctc ggggtccttc gacaagacgg
301ccagcgtctt cttgctggag aaggaccggt tggtcaaaga aaacaattat cggggacatg
361gggatagtgt ggaccagctt tgttggcatc caagtaatcc tgacctattt gttacggcgt
421ctggagataa aaccattcgc atctgggatg tgaggactac aaaatgcatt gccactgtga
481acactaaagg ggagaacatt aatatctgct ggagtcctga tgggcagacc attgctgtag
541gcaacaagga tgatgtggtg acctttattg atgccaagac acaccgttcc aaagcagaag
601agcagttcaa gttcgaggtc aacgaaatct cctggaacaa tgacaataat atgttcttcc
661tgacaaatgg caatggttgt atcaacatcc tcagctaccc agaactgaag cctgtgcagt
721ccatcaacgc ccatccttcc aactgcatct gtatcaagtt tgaccccatg gggaagtact
781ttgccacagg aagtgcagat gctttggtca gcctctggga tgtggatgag ttagtgtgtg
841ttcggtgctt ttccaggctg gattggcctg taagaaccct cagtttcagc catgatggga
901aaatgctggc gtcagcatcg gaagatcatt ttattgacat tgctgaagtg gagacagggg
961acaaactatg ggaggtacag tgtgagtctc cgaccttcac agtggcgtgg caccccaaaa
1021ggcctctgct ggcatttgcc tgtgatgaca aagacggcaa atatgacagc agccgggaag
1081ccggaactgt gaagctgttt gggcttccta atgattcttg cccaactttc ttgtacaaag
1141tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1201gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)