Construct: ORF ccsbBroad304_09240
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017014.1_s304c1, BRDN0000403241
- Derived from:
- ccsbBroadEn_09240
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG10 (84920)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09240
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84920 | ALG10 | ALG10 alpha-1,2-glucosyltra... | NM_032834.4 | 99.9% | 99.7% | 1416G>A |
| 2 | human | 144245 | ALG10B | ALG10 alpha-1,2-glucosyltra... | NM_001013620.4 | 97.6% | 95.7% | (many diffs) |
| 3 | human | 84920 | ALG10 | ALG10 alpha-1,2-glucosyltra... | XM_024449230.1 | 87.2% | 87.1% | 0_1ins180;1236G>A |
| 4 | human | 84920 | ALG10 | ALG10 alpha-1,2-glucosyltra... | XM_024449231.1 | 87.2% | 87.1% | 0_1ins180;1236G>A |
| 5 | human | 144245 | ALG10B | ALG10 alpha-1,2-glucosyltra... | XM_005268665.4 | 85.3% | 83.2% | (many diffs) |
| 6 | human | 144245 | ALG10B | ALG10 alpha-1,2-glucosyltra... | NM_001308340.2 | 25.5% | 24.9% | (many diffs) |
| 7 | mouse | 380959 | Alg10b | asparagine-linked glycosyla... | NM_001033441.3 | 82.8% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1485
- ORF length:
- 1419
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagctggaa ggttactatt tctcggccgc cttgagctgt acctttttag 121 tatcctgcct cctcttctcc gccttcagcc gggcgttgcg agagccctac atggacgaga 181 tcttccacct gcctcaggcg cagcgctact gtgagggcca tttctccctt tcccagtggg 241 atcccatgat tactacatta cctggcttgt acctggtgtc aattggagtg atcaaacctg 301 ccatttggat ctttggatgg tctgaacatg ttgtctgctc cattgggatg ctcagatttg 361 ttaatcttct cttcagtgtt ggcaacttct atttactata tttgcttttc tgcaaggtac 421 aacccagaaa caaggctgcc tcaagtatcc agagagtctt gtcaacatta acactagcag 481 tatttccaac actttatttt tttaacttcc tttattatac agaagcagga tctatgtttt 541 ttactctttt tgcgtatttg atgtgtcttt atggaaatca taaaacttca gccttccttg 601 gattttgtgg cttcatgttt cggcaaacaa atatcatctg ggctgtcttc tgtgcaggaa 661 atgtcattgc acaaaagtta acggaggctt ggaaaactga gctacaaaag aaggaagaca 721 gacttccacc tattaaagga ccatttgcag aattcagaaa aattcttcag tttcttttgg 781 cttattccat gtcctttaaa aacttgagta tgcttttgct tctgacttgg ccctacatcc 841 ttctgggatt tctgttttgt gcttttgtag tagttaatgg tggaattgtt attggcgatc 901 ggagtagtca tgaagcctgt cttcattttc ctcaactatt ctactttttt tcatttactc 961 tctttttttc ctttcctcat ctcctgtctc ctagcaaaat taagactttt ctttccttag 1021 tttggaaacg tagaattctg ttttttgtgg ttaccttagt ctctgtgttt ttagtttgga 1081 aattcactta tgctcataaa tacttgctag cagacaatag acattatact ttctatgtgt 1141 ggaaaagagt ttttcaaaga tatgaaactg taaaatattt gttagttcca gcctatatat 1201 ttgctggttg gagtatagct gactcattga aatcaaagtc aattttttgg aatttaatgt 1261 ttttcatatg cttgttcact gttatagttc ctcagaaact gctggaattt cgttacttca 1321 ttttacctta tgtcatttat aggcttaaca tacctctgcc tcccacatcc agactcattt 1381 gtgaactgag ctgctatgca gttgttaatt tcataacttt tttcatcttt ctgaacaaga 1441 cttttcagtg gccaaatagt caggacattc aaaggtttat atggtaccca actttcttgt 1501 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1561 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a