Construct: ORF ccsbBroad304_09395

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011270.1_s304c1, BRDN0000410279
Derived from:
ccsbBroadEn_09395
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EFHC1 (114327)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09395
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 114327 EFHC1 EF-hand domain containing 1 NM_018100.4 99.8% 99.6% 854G>T;1855A>C
2 human 114327 EFHC1 EF-hand domain containing 1 NM_001172420.2 96.5% 96.4% (many diffs)
3 human 114327 EFHC1 EF-hand domain containing 1 NR_033327.2 23.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1986
ORF length:
1920
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt gtccaatccc gtgcatggct tgccctttct tccgggcacg tcctttaagg
121actctacgaa aacagccttc cacagaagtc agacgctgag ctacaggaac ggctatgcaa
181ttgttcgacg tccaacagtt gggataggcg gagaccggct ccagttcaac cagctgtccc
241aggctgagct ggatgagttg gccagtaagg caccagtctt aacttatggc caacctaaac
301aagccccacc tgcggatttt attcctgcgc atgtggcctt tgacaaaaag gtactgaaat
361ttgatgccta tttccaagaa gatgttccta tgtcaactga ggaacagtat aggatccgtc
421aggtgaacat ttactattat ctagaagatg acagcatgtc tgtcatagag cctgttgtag
481aaaattctgg aatccttcaa ggcaagttaa taaaacgcca gcggctagcc aagaatgacc
541ggggtgacca ttaccattgg aaagacctaa atcgaggaat aaacatcaca atttatggca
601aaactttccg cgttgttgac tgtgaccaat tcacacaggt atttttagaa agccaaggaa
661ttgagttaaa tccaccagag aagatggctc ttgatcctta cactgaactc cgaaaacagc
721ctcttcgtaa gtatgtcacc ccatcagact ttgatcaact caagcaattt ctcacctttg
781acaaacaggt ccttcgattc tatgcaatct gggatgatac agacagcatg tatggtgaat
841gtcggaccta catcattcat tactatctta tggatgatac ggtggaaatt cgagaggtcc
901acgaacggaa tgatgggata gatcctttcc cactcctaat gaaccgccag cgtgtgccca
961aagttttggt ggaaaatgca aagaacttcc ctcagtgtgt gctagaaatc tctgaccaag
1021aagtgttgga atggtatact gctaaagact tcattgttgg gaagtcactc actatccttg
1081ggagaacttt cttcatttat gattgtgatc catttactcg acggtattac aaagagaagt
1141ttggaatcac tgatttacca cgtattgatg tgagcaagcg ggaaccacct ccagtaaaac
1201aggagttgcc tccttataac ggttttggac tagtggaaga ttctgctcag aattgttttg
1261ctctcattcc aaaagctcca aaaaaagacg ttattaaaat gctggtgaat gataacaagg
1321tgcttcgtta tttggctgta ctggaatccc ccatcccaga agacaaagac cgcagatttg
1381tcttctctta ctttctagct accgacatga tcagtatctt tgagcctcct gttcgcaatt
1441ctggtatcat tgggggcaag taccttggca ggactaaagt tgttaaacca tactctacag
1501tggacaaccc tgtctactat ggccccagtg acttcttcat tggtgctgtg attgaagtgt
1561ttggtcaccg gttcatcatc cttgatacag acgagtatgt tttgaaatac atggagagca
1621acgctgccca gtattcacca gaagcactcg cgtcaattca gaaccatgtc cgaaagcgag
1681aagcgcctgc tccagaagca gaaagcaagc aaactgaaaa ggatccaggc gtgcaggaat
1741tggaagcatt aatagacaca attcagaagc aactgaaaga tcactcatgc aaagacaaca
1801ttcgtgaggc atttcaaatt tatgacaagg aagcttcagg atatgtggac agagacatgt
1861tctttaaaat ctgtgaatcg cttaacgtcc cagtggatga ctccttggtt aaggagttac
1921tcaggatgtg ctctcatgga gaaggcaaaa ttaactacta taactttgtt cgtgctttct
1981caaactgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
2041gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
2101acctctggat ta

Download FASTA (ORF) (Full)