Construct: ORF ccsbBroad304_09395
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011270.1_s304c1, BRDN0000410279
- Derived from:
- ccsbBroadEn_09395
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFHC1 (114327)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09395
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114327 | EFHC1 | EF-hand domain containing 1 | NM_018100.4 | 99.8% | 99.6% | 854G>T;1855A>C |
| 2 | human | 114327 | EFHC1 | EF-hand domain containing 1 | NM_001172420.2 | 96.5% | 96.4% | (many diffs) |
| 3 | human | 114327 | EFHC1 | EF-hand domain containing 1 | NR_033327.2 | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1986
- ORF length:
- 1920
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gtccaatccc gtgcatggct tgccctttct tccgggcacg tcctttaagg 121 actctacgaa aacagccttc cacagaagtc agacgctgag ctacaggaac ggctatgcaa 181 ttgttcgacg tccaacagtt gggataggcg gagaccggct ccagttcaac cagctgtccc 241 aggctgagct ggatgagttg gccagtaagg caccagtctt aacttatggc caacctaaac 301 aagccccacc tgcggatttt attcctgcgc atgtggcctt tgacaaaaag gtactgaaat 361 ttgatgccta tttccaagaa gatgttccta tgtcaactga ggaacagtat aggatccgtc 421 aggtgaacat ttactattat ctagaagatg acagcatgtc tgtcatagag cctgttgtag 481 aaaattctgg aatccttcaa ggcaagttaa taaaacgcca gcggctagcc aagaatgacc 541 ggggtgacca ttaccattgg aaagacctaa atcgaggaat aaacatcaca atttatggca 601 aaactttccg cgttgttgac tgtgaccaat tcacacaggt atttttagaa agccaaggaa 661 ttgagttaaa tccaccagag aagatggctc ttgatcctta cactgaactc cgaaaacagc 721 ctcttcgtaa gtatgtcacc ccatcagact ttgatcaact caagcaattt ctcacctttg 781 acaaacaggt ccttcgattc tatgcaatct gggatgatac agacagcatg tatggtgaat 841 gtcggaccta catcattcat tactatctta tggatgatac ggtggaaatt cgagaggtcc 901 acgaacggaa tgatgggata gatcctttcc cactcctaat gaaccgccag cgtgtgccca 961 aagttttggt ggaaaatgca aagaacttcc ctcagtgtgt gctagaaatc tctgaccaag 1021 aagtgttgga atggtatact gctaaagact tcattgttgg gaagtcactc actatccttg 1081 ggagaacttt cttcatttat gattgtgatc catttactcg acggtattac aaagagaagt 1141 ttggaatcac tgatttacca cgtattgatg tgagcaagcg ggaaccacct ccagtaaaac 1201 aggagttgcc tccttataac ggttttggac tagtggaaga ttctgctcag aattgttttg 1261 ctctcattcc aaaagctcca aaaaaagacg ttattaaaat gctggtgaat gataacaagg 1321 tgcttcgtta tttggctgta ctggaatccc ccatcccaga agacaaagac cgcagatttg 1381 tcttctctta ctttctagct accgacatga tcagtatctt tgagcctcct gttcgcaatt 1441 ctggtatcat tgggggcaag taccttggca ggactaaagt tgttaaacca tactctacag 1501 tggacaaccc tgtctactat ggccccagtg acttcttcat tggtgctgtg attgaagtgt 1561 ttggtcaccg gttcatcatc cttgatacag acgagtatgt tttgaaatac atggagagca 1621 acgctgccca gtattcacca gaagcactcg cgtcaattca gaaccatgtc cgaaagcgag 1681 aagcgcctgc tccagaagca gaaagcaagc aaactgaaaa ggatccaggc gtgcaggaat 1741 tggaagcatt aatagacaca attcagaagc aactgaaaga tcactcatgc aaagacaaca 1801 ttcgtgaggc atttcaaatt tatgacaagg aagcttcagg atatgtggac agagacatgt 1861 tctttaaaat ctgtgaatcg cttaacgtcc cagtggatga ctccttggtt aaggagttac 1921 tcaggatgtg ctctcatgga gaaggcaaaa ttaactacta taactttgtt cgtgctttct 1981 caaactgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2041 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2101 acctctggat ta