Construct: ORF ccsbBroad304_09420
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006929.1_s304c1, BRDN0000461984
- Derived from:
- ccsbBroadEn_09420
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASGRP4 (115727)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09420
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115727 | RASGRP4 | RAS guanyl releasing protein 4 | NM_001146202.2 | 99.9% | 99.8% | 53T>C |
| 2 | human | 115727 | RASGRP4 | RAS guanyl releasing protein 4 | NM_170604.3 | 97.8% | 97.7% | 53T>C;510_551del |
| 3 | human | 115727 | RASGRP4 | RAS guanyl releasing protein 4 | NM_001146205.2 | 92.8% | 92.7% | 53T>C;510_551del;662_663ins102 |
| 4 | human | 115727 | RASGRP4 | RAS guanyl releasing protein 4 | NM_001146204.2 | 87.6% | 87.5% | 53T>C;510_551del;952_953ins207 |
| 5 | human | 115727 | RASGRP4 | RAS guanyl releasing protein 4 | NM_001146203.1 | 84.2% | 84.1% | 53T>C;510_551del;953_954ins276 |
| 6 | human | 115727 | RASGRP4 | RAS guanyl releasing protein 4 | NM_001146207.1 | 83.4% | 83.3% | 53T>C;510_551del;662_663ins291 |
| 7 | human | 115727 | RASGRP4 | RAS guanyl releasing protein 4 | XR_935732.2 | 77.5% | (many diffs) | |
| 8 | human | 115727 | RASGRP4 | RAS guanyl releasing protein 4 | NM_001146206.1 | 69.7% | 69.6% | 53T>C;510_551del;658_659ins567 |
| 9 | mouse | 233046 | Rasgrp4 | RAS guanyl releasing protein 4 | NM_145149.4 | 82% | 83.8% | (many diffs) |
| 10 | mouse | 233046 | Rasgrp4 | RAS guanyl releasing protein 4 | NM_001174155.1 | 81.3% | 83.1% | (many diffs) |
| 11 | mouse | 233046 | Rasgrp4 | RAS guanyl releasing protein 4 | XM_011250525.1 | 77.5% | 79.1% | (many diffs) |
| 12 | mouse | 233046 | Rasgrp4 | RAS guanyl releasing protein 4 | XM_017322163.1 | 72.7% | 74% | (many diffs) |
| 13 | mouse | 233046 | Rasgrp4 | RAS guanyl releasing protein 4 | XM_017322165.1 | 70.2% | 72.2% | (many diffs) |
| 14 | mouse | 233046 | Rasgrp4 | RAS guanyl releasing protein 4 | XM_017322164.1 | 69.7% | 71.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2046
- ORF length:
- 1977
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacagaaaa gacagtaaga ggaagtccca ccaggaatgc accggaaaaa 121 caggagggcg aggccggccc cgccaagtgc gccgccacaa gacatgcccc agccctcggg 181 aaatcagcaa ggtcatggct tccatgaacc tgggcctgct gagtgagggc ggctgcagcg 241 aagatgagct gctggagaaa tgcatccagt ccttcgattc agctggcagc ctgtgccacg 301 aggaccacat gctcaacatg gtgctggcca tgcacagctg ggtgctgccg tccgccgacc 361 tggctgcccg cctgctgacc tcataccaga aggccacagg ggacacccag gagctgagac 421 ggctgcagat ctgtcacctg gtcaggtact ggctgatgcg acaccctgag gtgatgcacc 481 aggatcccca gctagaagaa gtcataggtc gtttctgggc caccgtggcc cgggagggca 541 actcagccca gagaagactg ggagactctt ctgacctccc aggcctgggc aaaaagcgca 601 aagtgtcctt gcttttcgac cacttggaga cgggggagct ggctcagcac ctcacctacc 661 tggagttccg gtccttccag gctatcacgc cccaggacct gcggagctac gttttgcagg 721 gctcagtacg aggctgcccg gccctggagg gctccgtagg tctcagcaac agcgtgtccc 781 gctgggtgca ggtgatggtg ctgagccgtc ccgggcccct acagcgtgca caggtgctgg 841 acaagttcat tcacgtggca cagaggctcc accagctgca gaatttcaac acgctgatgg 901 cagtcacagg gggcctgtgt cacagtgcca tctccagact caaggactcc catgcccacc 961 tgagccctga cagcaccaag gccctcctgg agctcactga gctccttgcc tcccacaaca 1021 actacgcccg ctaccgccgc acctgggctg gctgcgcggg tttccggctg cctgtactgg 1081 gcgtgcacct caaggacctg gtgtccctgc atgaggcaca gcccgacagg ttgcctgacg 1141 gccgcctgca cctacccaag ctgaacaacc tctacctgcg gctgcaggag ctggtggccc 1201 tccaagggca gcatccaccc tgcagcgcca atgaggatct gctgcacctg ctcacgctct 1261 ccctggacct cttctacacg gaagacgaga tctatgagct ttcttatgcc cgggagccgc 1321 gttgtcccaa gagcctgcca ccctccccct tcaatgcacc tctggtggtg gagtgggccc 1381 ctggtgtgac acccaagccg gacagggtca cactgggtcg gcatgtggag cagctggtgg 1441 agtctgtgtt caagaattat gaccctgaag gccgaggaac aatctctcag gaggactttg 1501 agcgactctc gggcaatttt cccttcgcct gccatgggct tcacccaccc ccacgccagg 1561 ggagaggatc cttcagcaga gaggagctga cagggtacct gctccgggcc agcgccatct 1621 gctccaagtt gggcctggcc ttcctgcaca ccttccatga ggtcaccttc cgaaagccta 1681 ccttctgcga cagctgcagt ggcttcctct ggggtgtcac caagcaaggc taccgctgtc 1741 gggagtgcgg gctgtgttgc cacaaacact gcagagacca ggtgaaggta gaatgtaaga 1801 agaggccagg ggccaagggc gatgcaggac cccccggagc tcctgtccca tccacaccag 1861 ctccccatgc cagctgtggc tccgaggaaa atcactccta cacgctatcc ctggagcctg 1921 agactgggtg ccagcttcgc catgcctgga cccagactga atccccacac ccttcctggg 1981 aaacagatac ggtcccctgc ccggtgatgg acccaccatc aactgcatcc tccaagctgg 2041 attccttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2101 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2161 acctctggat ta