Construct: ORF ccsbBroad304_09524

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF006810.1_s304c1, BRDN0000407704
• Derived from: ccsbBroadEn_09524
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: CLHC1 (130162)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_09524
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	130162	CLHC1	clathrin heavy chain linker...	NM_001135598.1	99.8%	99.5%	167C>T;910G>A
2	human	130162	CLHC1	clathrin heavy chain linker...	NM_001353781.2	99.8%	99.5%	167C>T;910G>A
3	human	130162	CLHC1	clathrin heavy chain linker...	NM_001353784.2	99.8%	99.5%	167C>T;910G>A
4	human	130162	CLHC1	clathrin heavy chain linker...	NM_001353782.2	92.2%	90.7%	(many diffs)
5	human	130162	CLHC1	clathrin heavy chain linker...	NM_001353783.2	92.2%	90.7%	(many diffs)
6	human	130162	CLHC1	clathrin heavy chain linker...	NM_001353779.2	85.6%	85.3%	167C>T;334_335ins198;712G>A
7	human	130162	CLHC1	clathrin heavy chain linker...	NM_001353786.2	85.6%	85.3%	167C>T;334_335ins198;712G>A
8	human	130162	CLHC1	clathrin heavy chain linker...	NM_001353787.2	85.6%	85.3%	167C>T;334_335ins198;712G>A
9	human	130162	CLHC1	clathrin heavy chain linker...	NM_152385.4	79%	78.8%	1_366del;533C>T;1276G>A
10	human	130162	CLHC1	clathrin heavy chain linker...	NM_001353785.2	78.2%	76.5%	(many diffs)
11	human	130162	CLHC1	clathrin heavy chain linker...	NM_001353780.2	67.8%	67.5%	(many diffs)
12	human	130162	CLHC1	clathrin heavy chain linker...	NR_148541.2	23.8%		(many diffs)
13	human	130162	CLHC1	clathrin heavy chain linker...	NR_148540.2	23%		(many diffs)
14	human	130162	CLHC1	clathrin heavy chain linker...	NR_148543.2	21.3%		(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1458

ORF length:

1392

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatgag gattatcgaa agtaattcct cgaagattca atctcaaata gatcacatca
121	agcagtgtag ggcagagtat gacacaaaag aagtaaaata ttgtactttc tccaaagatc
181	cttcaaaacc tattccaggc atgactcttc aagaatccat gaatctagat gttctcacta
241	aatacatgaa acatcttgaa gataaatatg cagaaattaa acaagctatg ttgataaaat
301	atgtgccagc tcagaggaag gctgatttag atgaagaaat gattgtgtta ttaaaacggc
361	gagatgtagc tgaaaatctg aacaaaaaat tgcagttttg tcatcaaaga ctgcagataa
421	tttcacaggc acttagttca tgggtaaaat ctgatatgag cagcccattt caagactttg
481	tggagcaaat tcagaaaacc aaatatttac aaggtgacca aggcattgtt gaagaactaa
541	tggaagatga tccacgcagg gcaaaagaag ctgaaattat gcttcactac attgaaaggt
601	ttaatgagtt aatctcactt ggtgaatatg aaaaggcagc ttgttatgca gcaaacagtc
661	ctagaagaat tcttcgaaac attggtacaa tgaatacatt taaggctgtt ggaaaaatta
721	gaggaaagcc tcttccatta ctcttatttt ttgaggccct ctttatcaca agtcatgctt
781	ttccatgtcc tgttgatgca gctctaaccc tggaaggaat caaatgtgga ttatcagaaa
841	aacggttaga tttagttacc aattgggtta cacaggaaag actgacattt tctgaggagg
901	ctggggatgt gatttgtgat tatggggagc aggatactta taacaaggcc aagtgcctgg
961	ctttagctca gattatctac agtgaatgtg gcctgcacaa gaaagctatt ctttgcttgt
1021	gtaaacaggg tcagactcat agggtcatgg agtacataca gcagttgaag gactttacta
1081	ccgatgacct gttgcagcta ttaatgtcat gtccccaagt tgaattaatt cagtgtctca
1141	ctaaagagtt gaatgagaaa caaccatctt tatcttttgg tcttgctata cttcatctgt
1201	tctctgcaga catgaaaaaa gttggcatta agctacttca agaaatcaat aaaggtggga
1261	tagatgcagt agaaagtctt atgataaatg attccttttg ctccatagaa aagtggcaag
1321	aagtggcaaa tatatgttca cagaatggct ttgacaaatt atctaatgac atcacgtcta
1381	ttcttcgatc tcaggctgca gttacagaaa tttctgaaga ggatgacgca gtcaacctaa
1441	tggaacatgt gttttggtac ccaactttct tgtacaaagt ggttggtaag cctatcccta
1501	accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
1561	agtcgacaat caacctctgg atta

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