Construct: ORF ccsbBroad304_09525
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009338.1_s304c1, BRDN0000401126
- Derived from:
- ccsbBroadEn_09525
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLHC1 (130162)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09525
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_152385.4 | 99.8% | 99.6% | 84A>G;1276G>A;1508C>T |
| 2 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353780.2 | 88.5% | 88.3% | (many diffs) |
| 3 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001135598.1 | 79% | 78.8% | 0_1ins366;910G>A;1142C>T |
| 4 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353781.2 | 79% | 78.8% | 0_1ins366;910G>A;1142C>T |
| 5 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353784.2 | 79% | 78.8% | 0_1ins366;910G>A;1142C>T |
| 6 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353782.2 | 75.9% | 71.8% | (many diffs) |
| 7 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353783.2 | 75.9% | 71.8% | (many diffs) |
| 8 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353779.2 | 67.8% | 67.5% | (many diffs) |
| 9 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353786.2 | 67.8% | 67.5% | (many diffs) |
| 10 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353787.2 | 67.8% | 67.5% | (many diffs) |
| 11 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NM_001353785.2 | 64.7% | 60.5% | (many diffs) |
| 12 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NR_148541.2 | 23.6% | (many diffs) | |
| 13 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NR_148540.2 | 22.4% | (many diffs) | |
| 14 | human | 130162 | CLHC1 | clathrin heavy chain linker... | NR_148543.2 | 18% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1824
- ORF length:
- 1758
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc agttcatcaa ataagaaaac atgcagttct cccacctatc atttgtagaa 121 gtgacaagga atttttggaa agtgtgcaga gatacataat tacagaaact gaaagactgg 181 gctgtagtga ggaaggacct gctgatgaat attacatcat ataccgaaat gtttttgata 241 aggtaataga gcatatcact gcatacaaat ccattcttac ttcaatcaaa aaagaatatg 301 atgcctttat tgagacaata aagaaagacc gaagaactac attttgtctt catggaaaac 361 ttaaaggttt ggcagcagag cctacagctt tggtatatta caggaaaaga acaatccaac 421 ttgaagcaaa aatgaggatt atcgaaagta attcctcgaa gattcaatct caaatagatc 481 acatcaagca gtgtagggca gagtatgaca caaaagaagt aaaatattgt actttctcca 541 aagatccttc aaaacctatt ccaggcatga ctcttcaaga atccatgaat ctagatgctc 601 tcactaaata catgaaacat cttgaagata aatatgcaga aattaaacaa gctatgttga 661 taaaatatgt gccagctcag aggaaggctg atttagatga agaaatgatt gtgttattaa 721 aacggcgaga tgtagctgaa aatctgaaca aaaaattgca gttttgtcat caaagactgc 781 agataatttc acaggcactt agttcatggg taaaatctga tatgagcagc ccatttcaag 841 actttgtgga gcaaattcag aaaaccaaat atttacaagg tgaccaaggc attgttgaag 901 aactaatgga agatgatcca cgcagggcaa aagaagctga aattatgctt cactacattg 961 aaaggtttaa tgagttaatc tcacttggtg aatatgaaaa ggcagcttgt tatgcagcaa 1021 acagtcctag aagaattctt cgaaacattg gtacaatgaa tacatttaag gctgttggaa 1081 aaattagagg aaagcctctt ccattactct tattttttga ggccctcttt atcacaagtc 1141 atgcttttcc atgtcctgtt gatgcagctc taaccctgga aggaatcaaa tgtggattat 1201 cagaaaaacg gttagattta gttaccaatt gggttacaca ggaaagactg acattttctg 1261 aggaggctgg ggatgtgatt tgtgattatg gggagcagga tacttataac aaggccaagt 1321 gcctggcttt agctcagatt atctacagtg aatgtggcct gcacaagaaa gctattcttt 1381 gcttgtgtaa acagggtcag actcataggg tcatggagta catacagcag ttgaaggact 1441 ttactaccga tgacctgttg cagctattaa tgtcatgtcc ccaagttgaa ttaattcagt 1501 gtctcactaa agagttgaat gagaaacaac catctttatc ttttggtctt gctatacttc 1561 atctgttctc tgtagacatg aaaaaagttg gcattaagct acttcaagaa atcaataaag 1621 gtgggataga tgcagtagaa agtcttatga taaatgattc cttttgctcc atagaaaagt 1681 ggcaagaagt ggcaaatata tgttcacaga atggctttga caaattatct aatgacatca 1741 cgtctattct tcgatctcag gctgcagtta cagaaatttc tgaagaggat gacgcagtca 1801 acctaatgga acatgtgttt tggtacccaa ctttcttgta caaagtggtt ggtaagccta 1861 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1921 gcgttaagtc gacaatcaac ctctggatta