Construct: ORF ccsbBroad304_09633
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015824.1_s304c1, BRDN0000461353
- Derived from:
- ccsbBroadEn_09633
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFCAB3 (146779)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09633
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146779 | EFCAB3 | EF-hand calcium binding dom... | NM_173503.4 | 99.9% | 99.7% | 1146G>T |
| 2 | human | 146779 | EFCAB3 | EF-hand calcium binding dom... | XM_011524380.1 | 99.9% | 99.7% | 1146G>T |
| 3 | human | 146779 | EFCAB3 | EF-hand calcium binding dom... | XM_011524381.2 | 95.1% | 95% | 1_66del;1212G>T |
| 4 | human | 146779 | EFCAB3 | EF-hand calcium binding dom... | NM_001144933.1 | 89.3% | 89.1% | 1_156del;1302G>T |
| 5 | human | 102724660 | LOC102724660 | uncharacterized LOC102724660 | XR_927946.3 | 30.7% | (many diffs) | |
| 6 | human | 102724660 | LOC102724660 | uncharacterized LOC102724660 | XR_428246.3 | 30.1% | (many diffs) | |
| 7 | human | 102724660 | LOC102724660 | uncharacterized LOC102724660 | XR_927947.2 | 30% | (many diffs) | |
| 8 | human | 102724660 | LOC102724660 | uncharacterized LOC102724660 | XR_927948.2 | 29.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1383
- ORF length:
- 1314
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagtttca gaaattaaac caaaacttaa gctgaatcct ctaacaaaag 121 tacccatctc ccacaataaa agggatagag acttaccagg atctcttcaa tgccaattac 181 aacacaaaga aaagaagcta agtgcttcac aaatggcagc tttccaagat gcctacaact 241 tcttctacaa ggacaaaact ggctgtattg atttccatgg actgatgtgc actgtagcta 301 agctgggaat gaatctgacc aagcatgatg tctataatga attgaaatgt gctgatattg 361 atcgagatgg gaaggtgaac ttctcagact ttatcaaggt tctaacagat aagaatctct 421 tcctcaaggc agtggttcca gaaaaggaga cctgtttaga tttggctggc aacccaggaa 481 tcctattgtt tgaaatccta tcaaggcttc tagagacttc agccctaccc agaaagtcta 541 taatagaaat agtaagctat ttccaaagaa aattccagca tactggccca ggaatgttgt 601 ggagtcccta cactatgggc tatggaaaaa ggacacttaa gccagacata tgcacacctc 661 caagctcaag catggctgcc tttgctaatg ctgcccggat tgcaataatg aaagaaaagg 721 atttatttaa atttcttgaa gagctcaaga gatgcaattc cggttcagat agcccatatt 781 caaaaatacc catctttcca ttgttcccta atgtggatgg ggtggtgatg ggaaagccat 841 tcaaagacat gcagaaatta gagatgctaa gaataaagga gcctttgcat ttctttgagg 901 attatttttt ccataaaaga gactggaaaa cacaggcagc aaatataaag tctatggacc 961 ctgcctcagg ttattcaaat aacatcttca ccattgatca aatgctcaag aaaaagcaga 1021 cttgtacagt ggccgatgca actgctatta aacaacatgt gaagagagct actgatacct 1081 ataatttagg aattgccctt gaacaccgaa aagagatgct aaacctctgg cagaagatcc 1141 gaggggattt gattgggatg gactctagaa atgagtcctt ttatgacacc ttttctactt 1201 atacatggtc ctgtaatgtt tgccaagaat tactttctcc taaggactta aggttatatg 1261 atgcctatgt gaatagaaat tcctcccata actccagatc ctcttcctca tcagatacca 1321 gtgaatgtta cacagactca ggaagaaaaa gaaaacggaa aggtttaaag ggatttcaac 1381 aattgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1441 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1501 tctggatta