Construct: ORF ccsbBroad304_09637
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008995.1_s304c1, BRDN0000408343
- Derived from:
- ccsbBroadEn_09637
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UNC45B (146862)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09637
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146862 | UNC45B | unc-45 myosin chaperone B | NM_001033576.2 | 99.9% | 99.8% | 178G>A |
| 2 | human | 146862 | UNC45B | unc-45 myosin chaperone B | NM_001267052.2 | 99.9% | 99.8% | 178G>A |
| 3 | human | 146862 | UNC45B | unc-45 myosin chaperone B | NM_173167.3 | 99.7% | 99.6% | 178G>A;1690_1695delGCAGGT |
| 4 | human | 146862 | UNC45B | unc-45 myosin chaperone B | NM_001308281.1 | 91.4% | 91.3% | 178G>A;1451_1452ins237 |
| 5 | human | 146862 | UNC45B | unc-45 myosin chaperone B | XM_017024234.1 | 91.4% | 91.3% | 178G>A;1451_1452ins237 |
| 6 | mouse | 217012 | Unc45b | unc-45 myosin chaperone B | NM_178680.4 | 88.7% | 95% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2856
- ORF length:
- 2787
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagaggtg gaagcggtac agctgaagga ggaaggaaac cggcatttcc 121 agctccagga ctacaaggcc gccacaaata gctacagcca ggccctgaag ctgaccaagg 181 acaaggccct gctggccacg ctttatcgga accgggcagc ctgtggcctg aaaacggaga 241 gctacatcca ggcagcttca gatgcctcca gagccatcga catcaactcc tcggacatca 301 aggctctgta tcggcgatgc caggcactgg agcacctggg gaagctggac caggccttca 361 aagacgtgca gcgttgtgcc accctcgagc cacggaacca gaacttccag gagatgctga 421 ggagactcaa caccagcatt caggagaagc tccgagtgca gttctccaca gactcgaggg 481 tacagaagat gtttgagatc ctcttggatg aaaacagtga ggctgataag cgggaaaagg 541 ctgccaacaa tctcattgtc ctaggccgtg aggaagcagg ggctgagaag atcttccaga 601 acaatggagt agccttgcta ctgcagcttc tggacactaa gaagcctgag ctggtgctgg 661 ctgcagtgcg gaccctgtcg ggcatgtgca gcggccacca agccagagcc acagtgattc 721 tgcatgcagt gcggatagac cgaatctgta gcctcatggc cgtggagaat gaggagatgt 781 ctctggctgt ctgcaacctg ctccaagcca tcattgactc cttgtctggg gaggacaagc 841 gggagcatcg agggaaggag gaggccctgg ttctagacac caagaaggac ctgaagcaga 901 tcaccagcca cctgctggac atgctagtca gcaagaaggt gtctggccag ggcagggatc 961 aggcgctgaa cctgctcaat aagaatgttc ccaggaagga ccttgccatt catgacaact 1021 cacgtaccat ctatgtggtg gataatggtc tgaggaagat cctgaaggtt gtggggcagg 1081 ttccagatct gccatcctgc ctgcccctga ctgacaacac ccgcatgctg gcctctatcc 1141 tcatcaacaa gctctatgat gacctgcgct gtgacccgga gcgcgatcac ttccgcaaga 1201 tctgtgagga atatatcacg ggcaagtttg acccccagga catggacaag aacttgaatg 1261 ccatccagac agtgtcaggg atcctgcagg gcccctttga cctgggcaac cagctgctgg 1321 gactgaaagg tgtgatggag atgatggtgg cactatgtgg ctcagagcgc gagacggacc 1381 agctggtggc cgtggaggcc ctcatccatg cctccacgaa gctcagccgc gccaccttca 1441 tcatcaccaa tggagtgtca ctgctcaaac agatctacaa gaccaccaaa aatgagaaga 1501 tcaagatccg cacactggtg ggactctgta agctcggctc tgcaggtggc acagactacg 1561 gtctcaggca gtttgcggaa gggtcgacag aaaaactggc caaacagtgt cgcaagtggc 1621 tgtgcaatat gtccatagac actcggaccc gacgctgggc agtggagggc ctggcctacc 1681 tcacgctgga cgctgatgtg aaggacgact ttgtccagga cgtccctgcc ctgcaggcca 1741 tgtttgagct ggccaagacc agtgacaaga ccatcctgta ctcggtggcc accaccctgg 1801 tgaactgcac caacagctac gatgtcaagg aggtcatccc agagcttgtc cagctcgcca 1861 agttctccaa gcagcatgtg cccgaggaac accccaagga caagaaggac tttatagaca 1921 tgcgggtgaa gcggcttctg aaggcgggtg tcatctctgc cctggcttgc atggtgaaag 1981 cagatagtgc catcctcact gaccagacca aggagctgct ggccagggta ttcctggcac 2041 tgtgtgacaa cccaaaggac cgaggcacca ttgtggctca aggtggtggc aaggccctga 2101 ttcccctggc tttggagggc acagatgtgg gcaaggtgaa ggcagcccac gctctagcaa 2161 agatcgctgc tgtctccaat ccggacattg cttttcctgg ggagcgggtg tatgaggtgg 2221 tgcggcccct tgtaagactc ttggacacac agagggatgg gcttcagaac tatgaggctc 2281 tcctaggcct caccaacctg tctgggcgga gtgacaaact ccggcagaag atctttaagg 2341 agagggcctt gccagacatc gagaactaca tgtttgagaa tcatgatcag ctgcggcagg 2401 cggccaccga gtgcatgtgc aacatggtgc tccacaagga ggtacaggaa aggttcttgg 2461 ctgacgggaa tgaccggctg aagctggtgg tgctgctctg cggggaggat gatgataagg 2521 tgcagaatgc ggctgcaggg gctctggcca tgctgacagc agcacacaag aaactgtgcc 2581 tcaagatgac tcaagtgaca acccagtggt tggagatcct ccagcggctt tgcctgcacg 2641 accagctgtc tgtccaacac cggggcctgg tcattgccta caacctactg gcagccgatg 2701 ctgagctggc caagaagctg gtggagagtg agctgctgga gatcctgact gtggtgggca 2761 aacaggagcc agatgagaag aaggcagaag tggttcagac agcccgagaa tgtctcatca 2821 agtgcatgga ttatggtttc attaaaccag tgtctttgcc aactttcttg tacaaagtgg 2881 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2941 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta