Transcript: Human NM_001267052.2

Homo sapiens unc-45 myosin chaperone B (UNC45B), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-09
Taxon:
Homo sapiens (human)
Gene:
UNC45B (146862)
Length:
5668
CDS:
95..2884

Additional Resources:

NCBI RefSeq record:
NM_001267052.2
NBCI Gene record:
UNC45B (146862)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001267052.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000164143 CGTACCATCTATGTGGTGGAT pLKO.1 1049 CDS 100% 2.640 3.696 N UNC45B n/a
2 TRCN0000159504 CGAGAACTACATGTTTGAGAA pLKO.1 2386 CDS 100% 4.950 3.960 N UNC45B n/a
3 TRCN0000164173 CTTGCCAGACATCGAGAACTA pLKO.1 2374 CDS 100% 4.950 3.960 N UNC45B n/a
4 TRCN0000418574 CAAGATCTGTGAGGAATATAT pLKO_005 1222 CDS 100% 15.000 10.500 N UNC45B n/a
5 TRCN0000434577 TGACAATGAAGTCTCAATATA pLKO_005 2985 3UTR 100% 15.000 10.500 N UNC45B n/a
6 TRCN0000158539 CTCATCAACAAGCTCTATGAT pLKO.1 1166 CDS 100% 5.625 3.938 N UNC45B n/a
7 TRCN0000163938 GCCAGACATCGAGAACTACAT pLKO.1 2377 CDS 100% 4.950 3.465 N UNC45B n/a
8 TRCN0000161125 GCCAACAATCTCATTGTCCTA pLKO.1 569 CDS 100% 2.640 1.848 N UNC45B n/a
9 TRCN0000163436 GCCATTCATGACAACTCACGT pLKO.1 1031 CDS 100% 2.640 1.848 N UNC45B n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001267052.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09637 pDONR223 100% 99.9% 99.8% None 178G>A n/a
2 ccsbBroad304_09637 pLX_304 0% 99.9% 99.8% V5 178G>A n/a
3 TRCN0000471965 CTTAATAAGGCATCGCCATATAAT pLX_317 18.9% 99.9% 99.8% V5 178G>A n/a
4 ccsbBroadEn_13235 pDONR223 100% 91.4% 91.4% None 381G>A;1452_1688del n/a
5 ccsbBroad304_13235 pLX_304 0% 91.4% 91.4% V5 381G>A;1452_1688del n/a
6 TRCN0000475781 ATTGCCAAAGTATATTTTTTTACC pLX_317 14.3% 91.4% 91.4% V5 381G>A;1452_1688del n/a
Download CSV