Construct: ORF ccsbBroad304_09708
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007607.1_s304c1, BRDN0000411841
- Derived from:
- ccsbBroadEn_09708
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNLDC1 (154197)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09708
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154197 | PNLDC1 | PARN like, ribonuclease dom... | NM_173516.2 | 99.9% | 100% | 231T>G |
| 2 | human | 154197 | PNLDC1 | PARN like, ribonuclease dom... | XM_011535491.2 | 99.9% | 100% | 231T>G |
| 3 | human | 154197 | PNLDC1 | PARN like, ribonuclease dom... | NM_001271862.2 | 96.3% | 95.6% | (many diffs) |
| 4 | human | 154197 | PNLDC1 | PARN like, ribonuclease dom... | XM_017010316.1 | 92.6% | 92.5% | 101_102ins114;117T>G |
| 5 | human | 154197 | PNLDC1 | PARN like, ribonuclease dom... | XM_017010312.2 | 91% | 87.6% | (many diffs) |
| 6 | human | 154197 | PNLDC1 | PARN like, ribonuclease dom... | XM_017010315.2 | 89.2% | 88.3% | (many diffs) |
| 7 | human | 154197 | PNLDC1 | PARN like, ribonuclease dom... | XM_017010313.2 | 84.3% | 80.9% | (many diffs) |
| 8 | human | 154197 | PNLDC1 | PARN like, ribonuclease dom... | XM_017010314.2 | 82.5% | 79.3% | (many diffs) |
| 9 | human | 154197 | PNLDC1 | PARN like, ribonuclease dom... | XM_011535493.3 | 76.3% | 73.6% | (many diffs) |
| 10 | human | 154197 | PNLDC1 | PARN like, ribonuclease dom... | XM_024446339.1 | 43.3% | 40.7% | (many diffs) |
| 11 | mouse | 240023 | Pnldc1 | poly(A)-specific ribonuclea... | XM_011246213.1 | 48.7% | 48.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1629
- ORF length:
- 1560
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gttctgcacc cgaggactgc tattttttgc cttcctggca ggtctggaca 121 tagagttcac gggccttcgt tctaacctgt ctgggcccca gcagatcagt ctttttgatt 181 tgccatcgga gtggtatcta aagacccgtc agagtgttca gcaatttaca gtctgtcaga 241 ttggattgtc tgtgttttcc gctattgaag gagaggcaaa caagtatata gcccattcgt 301 gtaacttcta tctcttccct acaacgtttg ggattttgga ctcagaattc tccttccagg 361 cttccagtgt tcagtttttg aatcagtatg gcttcaacta taacaagttt ctcaaaaacg 421 gaatcccata tatgaatgaa gaacaggaga agaaaattag acacgatatc ctgactggga 481 actggagagt tcgcagctct ccggataaag accaaatcaa ggtggtgatt gacgaagtga 541 cgcggtggct ggagctggcc aaggaaggcg actggatgac tcttcctggg atcactggct 601 tccaggcctt tgaggtccaa ctggtgctga ggcaggccct ccccaacatc tggacggtgc 661 tgaaagatga gggggtggta gtgaagaaag tgagtaaaca acatcgttgg tatcttcaga 721 acacctcttg tgaccgagag agctgttgga aggaaaatat tcttctctca gcaaggggtt 781 tttctgtctt tttccaaatg ctggtgaaag cccagaagcc cttagtggga cataatatga 841 tgatggacct gctgcacctc catgagaagt tcttcagacc cctcccagaa agctacgatc 901 aatttaagca gaatatccac agcctatttc ctgttctcat tgataccaag agtgtaacaa 961 aggatatctg gaaggagatg aatttcccga gggtgtcgaa tctttcggaa gtctatgaag 1021 tcctgaacag tgacttgaat cccaccaaga attctggacc agagattgtt cacgcgagca 1081 ggtgtgagaa atatgttgag acaaagtgcc cccacgaagc cgcgtatgat gccttcctct 1141 gtgggtcagt tcttttgaaa gtggcacact tgcttctaca gaagatatac cacatcgacc 1201 ccgtgcccga gtcatccttt cctcagtacc ttgacgtgct ggctccttac gtgaaccaag 1261 tgaacctcat ccgagcgggg gtcccaaaga tcaatttttc tggtccagat tatcccagta 1321 tccgacctcc catcctcatc ctcagcgtca aaaggtggcc tggggtcagc gagcagcaag 1381 tctaccataa gtttcagaat ctctgcaagt ttgatgtcag gcgactcaca agaagccagt 1441 tcttactcct gaccaataag tttaaggatg cgcggaacat cctgaaggag taccgggacc 1501 acccgaccct gtgcatctcc ctgtaccgct actggaggca ctccccaaac gtcaactgcc 1561 tgctccaagt ctgtggcata gtgactgcct gggcccttct cgcgttcatc cttggaagat 1621 ctggtacctt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1681 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1741 tcaacctctg gatta