Construct: ORF ccsbBroad304_09753
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016828.1_s304c1, BRDN0000461865
- Derived from:
- ccsbBroadEn_09753
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LEXM (163747)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09753
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163747 | LEXM | lymphocyte expansion molecule | NM_152607.3 | 99.9% | 99.7% | 376G>T |
| 2 | human | 163747 | LEXM | lymphocyte expansion molecule | XM_017000481.1 | 95.2% | 95.1% | 376G>T;1185_1185delGins58 |
| 3 | human | 163747 | LEXM | lymphocyte expansion molecule | NM_001110533.1 | 94.4% | 95.2% | (many diffs) |
| 4 | human | 163747 | LEXM | lymphocyte expansion molecule | XM_017000480.1 | 92.2% | 91.7% | (many diffs) |
| 5 | human | 163747 | LEXM | lymphocyte expansion molecule | XR_001737006.1 | 79.8% | (many diffs) | |
| 6 | human | 163747 | LEXM | lymphocyte expansion molecule | XR_001737007.1 | 76.5% | 1_54del;430G>T;1297_1622del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1311
- ORF length:
- 1242
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagggaaagc caggatgccg ccggagctca tggctggaac cgcgtcggct 121 ccacggccac caagtggttc accggggcgc ccttcggggt gcagagccac aggtttgaca 181 tctctgctgt ttatcccaac tggaagaagt tcagcacctt cactgaggcc ccatactcca 241 cgcgttattc tacccaagtg tcccacatag gccctgggac ttacagctcc aaagagacct 301 gcttcagcaa gaagaagctg atgaaggagg tggacacagg ctgggccaag gcccaggaag 361 ccacgcggct gacccagcta ccccacttcc agtaccaggc catcatgaaa gagaagcggc 421 tgaaggagca aaagctgggc ccctgctcct acaacctcaa agacttctta gaacagctgc 481 gggagaaacc atgtagcacc cgggggctgc tcagctctgg ggaggttcgc ttccgaggac 541 tcactgggaa ctactatcca ggccctggaa attatgggga gaagggtaac ccatacacca 601 agctggagga gaatgcctgg aaccggtctc attccgaggg cctcatgtgc aggatgagca 661 acaagccaca cccccggcct catcagggga gtggtctggg acccggcacc tacttcttca 721 aaagcgacct tgagacatat gtggcacgat ccgtcggcac ccgcggcccc tatgacactt 781 tctctggtga tcggagcaag ccactgcctt atgggcacta ctccatgcag aaaaaaaagc 841 ccagggaact gatgaatttc aagagctttg tagaagaact taactcacat cacaataaga 901 agcatggggt tttttctaaa cttccccgaa acccgaaaac ccctacagag aggatttact 961 gggccaacct cagccagtgc ccccgcacac tggccacatc tggccccagt ttctggcttc 1021 cacaagagaa gaaatgcaaa cccgtcaacc agcccccatt cctgttgacc tccaaggggt 1081 caggtgcaaa ggcctgccag atgattatgg gaagctggaa cccagtaggt gtgggccgct 1141 acctcaacac ctggctgatg gagacaaagg acaggcggca gcgatatcga tccctattcc 1201 tgagtggatc caaacgctac ctctcagacc tggcccggga catgctcatg caaatcaggc 1261 tgttttgttg gaaaggccta gaagtcatca attttggcct ctcaccaatg ttgccaactt 1321 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1381 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta