Construct: ORF ccsbBroad304_10232

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011904.1_s304c1, BRDN0000409170
Derived from:
ccsbBroadEn_10232
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDIA5 (10954)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10232
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10954 PDIA5 protein disulfide isomerase... NM_006810.4 50.1% 49.7% (many diffs)
2 human 10954 PDIA5 protein disulfide isomerase... NR_028444.2 46.5% 1_140del;927_1688del
3 human 10954 PDIA5 protein disulfide isomerase... XR_427360.1 46% 1_140del;927_1707del
4 human 10954 PDIA5 protein disulfide isomerase... XR_427359.1 42.7% (many diffs)
5 mouse 72599 Pdia5 protein disulfide isomerase... XR_384605.3 49.6% (many diffs)
6 mouse 72599 Pdia5 protein disulfide isomerase... XR_384603.3 44.6% (many diffs)
7 mouse 72599 Pdia5 protein disulfide isomerase... NM_028295.1 42.8% 44.6% (many diffs)
8 mouse 72599 Pdia5 protein disulfide isomerase... XR_384604.3 39.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
852
ORF length:
786
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gcgggccggg ccggcgtggc tgctgctggc aatctgggtg gtcctgccat
121catggctgtc ctctgcaaag gtctcctcgc tcattgagag aatctctgac cccaaggact
181tgaaaaaact gctcagaacc cggaataatg tactggtgct ttactccaaa tctgaggtgg
241cagctgaaaa tcatctcagg ttactgtcca cagtggccca ggcggtgaaa ggacaaggga
301ccatctgctg ggtggactgt ggtgatgcag agagtagaaa attgtgcaag aagatgaaag
361ttgacctgag cccgaaggac aaaaaggttg aattattcca ttaccaggat ggtgcatttc
421atactgaata taaccgagct gtgacattta agtccatagt ggcctttttg aaggatccaa
481aagggccccc actgtgggag gaagatcctg gagccaaaga tgttgtccac cttgacagtg
541aaaaggactt cagacggctc ctgaagaagg aagagaagcc gctcctgatc atgttttatg
601ccccctggtg cagcatgtgc aagaggatga tgccgcattt ccagaaggct gcgactcagc
661tgcgaggcca cgccgtgctg gccgggatga atgtctactc ctctgaattt gaaaacatca
721aggaggagta cagcgtgcgc ggcttcccca ccatctgcta ttttgagaaa ggacggttct
781tgttccagta tgacaactat gggtccacag ctgaggacat tgtggagtgg ctgaagaagg
841tgtggccact gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
901tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
961caatcaacct ctggatta

Download FASTA (ORF) (Full)