Construct: ORF ccsbBroad304_10264
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011027.1_s304c1, BRDN0000403328
- Derived from:
- ccsbBroadEn_10264
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFSD14C (84278)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10264
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84278 | MFSD14C | major facilitator superfami... | NM_001355228.1 | 56.6% | 56.6% | 1_195del |
| 2 | human | 84278 | MFSD14C | major facilitator superfami... | XM_024447698.1 | 49.5% | 42.5% | (many diffs) |
| 3 | human | 84278 | MFSD14C | major facilitator superfami... | NM_001355230.1 | 47.1% | 45.3% | (many diffs) |
| 4 | human | 84278 | MFSD14C | major facilitator superfami... | XM_024447700.1 | 46% | 44.6% | (many diffs) |
| 5 | human | 84278 | MFSD14C | major facilitator superfami... | XM_024447699.1 | 45.9% | 42.4% | (many diffs) |
| 6 | human | 84278 | MFSD14C | major facilitator superfami... | NM_001355229.2 | 44.1% | 43.5% | (many diffs) |
| 7 | human | 84641 | MFSD14B | major facilitator superfami... | XM_017015221.1 | 26.1% | 21.6% | (many diffs) |
| 8 | human | 84641 | MFSD14B | major facilitator superfami... | XM_011519114.2 | 18.4% | 15.3% | (many diffs) |
| 9 | human | 84641 | MFSD14B | major facilitator superfami... | XM_017015217.2 | 18.4% | 15.3% | (many diffs) |
| 10 | human | 84641 | MFSD14B | major facilitator superfami... | XM_017015218.1 | 18.4% | 15.3% | (many diffs) |
| 11 | human | 84641 | MFSD14B | major facilitator superfami... | XM_017015219.2 | 18.3% | 15.5% | (many diffs) |
| 12 | human | 84641 | MFSD14B | major facilitator superfami... | NM_032558.3 | 16% | 13.3% | (many diffs) |
| 13 | human | 84278 | MFSD14C | major facilitator superfami... | XR_002956812.1 | 12.3% | (many diffs) | |
| 14 | human | 84641 | MFSD14B | major facilitator superfami... | XR_002956813.1 | 7.6% | (many diffs) | |
| 15 | mouse | 66631 | Mfsd14b | major facilitator superfami... | NM_001083901.1 | 14.3% | 12.9% | (many diffs) |
| 16 | mouse | 66631 | Mfsd14b | major facilitator superfami... | NM_133680.3 | 14.3% | 12.9% | (many diffs) |
| 17 | mouse | 66631 | Mfsd14b | major facilitator superfami... | XM_011244550.2 | 14.3% | 12.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 321
- ORF length:
- 255
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt gactgttcta catgaaacat tttctcaaca cacattcctc atgaatggtc 121 tcattcaagg tgtaaagggc ctgctctctt ttttgagtgc cccactcatt ggtgccctgt 181 ctgatgtgtg ggggaggaag ccctttctcc tcggcactgt attctttacc tgcttcccaa 241 tcccactgat gaggatcagc ccatgccggg tgtggtggcg tgcacctgta gtcccagcta 301 cctgtggcag gagaatggct tgcccaactt tcttgtacaa agtggttggt aagcctatcc 361 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 421 ttaagtcgac aatcaacctc tggatta