Construct: ORF ccsbBroad304_10395
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000141.1_s304c1, BRDN0000398664
- Derived from:
- ccsbBroadEn_10395
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIR4435-2HG (541471)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10395
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112597 | CYTOR | cytoskeleton regulator RNA | NR_024206.2 | 28.2% | (many diffs) | |
| 2 | human | 112597 | CYTOR | cytoskeleton regulator RNA | NR_146460.1 | 26.7% | (many diffs) | |
| 3 | human | 541471 | MIR4435-2HG | MIR4435-2 host gene | NR_024373.2 | 25.8% | 1_325del;482_603del | |
| 4 | human | 112597 | CYTOR | cytoskeleton regulator RNA | NR_024205.3 | 22.5% | (many diffs) | |
| 5 | human | 112597 | CYTOR | cytoskeleton regulator RNA | NR_146461.1 | 20.9% | (many diffs) | |
| 6 | human | 112597 | CYTOR | cytoskeleton regulator RNA | NR_024204.2 | 17.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 222
- ORF length:
- 156
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac agacactgaa aatcacgact catccccctc cagcacctct acctgttgcc 121 cgccgatcac agccggaatg cagctgaaag attccctggg gcctggttcc aactgcccac 181 tgtggactct gaggcctctg catttgcggg tggtctgcct gtgcccaact ttcttgtaca 241 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 301 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta