Construct: ORF ccsbBroad304_10529
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013052.1_s304c1, BRDN0000401370
- Derived from:
- ccsbBroadEn_10529
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIR22HG (84981)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10529
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84981 | MIR22HG | MIR22 host gene | NR_028505.1 | 12.5% | 1_311del;373T>C;483_1356del | |
| 2 | human | 84981 | MIR22HG | MIR22 host gene | NR_028504.1 | 11.8% | 1_394del;456T>C;566_1439del | |
| 3 | human | 84981 | MIR22HG | MIR22 host gene | NR_028503.1 | 8.6% | (many diffs) | |
| 4 | human | 84981 | MIR22HG | MIR22 host gene | NR_028502.1 | 6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 240
- ORF length:
- 171
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggctgggaa ggtccgaaca gcagggtgga tgatacgttt tgggcaagtt 121 ggagagcctc tgcccagatt ggcccagcaa ggagcggttt tagattagag acactggctg 181 gattgaggag tagaaggctc aaacaaccca aggctttctg cctccgagat gtggcaccat 241 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 301 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 361 ggatta