Construct: ORF ccsbBroad304_10559
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007565.1_s304c1, BRDN0000411584
- Derived from:
- ccsbBroadEn_10559
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOL4L-DT (149950)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10559
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 149950 | NOL4L-DT | NOL4L divergent transcript | NR_034152.1 | 48.3% | 1_232del;622A>G;653_867del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 489
- ORF length:
- 420
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggattgtaaa tccccaaagc gtgcaaacat ctgcccccat ctccctggag 121 gtggcctgtt ctccacgcca ccctcacagg ctgcctggcg gactctcctc acagcactgt 181 gcttcccggg gcccacttgt acaggcccca tgcgagaagg acctcgggca gtgtataatc 241 ctccaagggc ccacaggaac agcagtgaca actgtgttat gaagcaccta ctatgtgctg 301 gggacaagaa tggaacaaga agacatgctt tgccctcacc cctggagggc agcttccagc 361 cagggaggca aatacctcct ccccagactc cttccacaga tccccaaact ctgcctctct 421 ccttcagatc cctgctcaga tgtcaccagc tctgtgcggc ctccctgcca ccaagtctaa 481 aattgccatt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 541 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 601 tcaacctctg gatta