Construct: ORF ccsbBroad304_10560
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011878.1_s304c1, BRDN0000400399
- Derived from:
- ccsbBroadEn_10560
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ZNF295-AS1 (150142)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10560
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150142 | ZNF295-AS1 | ZNF295 antisense RNA 1 | NR_027273.2 | 52.7% | 1_167del;261T>C;579_777del | |
| 2 | human | 150142 | ZNF295-AS1 | ZNF295 antisense RNA 1 | NR_119385.1 | 49.8% | 1_213del;307T>C;625_823del | |
| 3 | human | 150142 | ZNF295-AS1 | ZNF295 antisense RNA 1 | NR_119384.1 | 40.7% | 1_396del;490T>C;808_1006del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 477
- ORF length:
- 411
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa agataaaatg tggtgtgagg acacggccca gccccaccgt cgtcttccag 121 ccccaccatc gtcttccagc cccaccgtcg tcttccagcc ccaccgtcgt cttctagccc 181 caccatcgtc gtcttggact ctctgggtgg caccacgctg tgggtgctgc tctcctctgt 241 gtcccaaacg agtttgtgcc tcactttgtc gggttttggc tgtgacttgg agcctcacca 301 agctcccttt tcctctgtct cccattctcc tctccaggcc agggtggctt ccccagccct 361 cctccccggg tcctgcctgt gccgagcctt cctcccagga gggaggtgat actgacttga 421 gatcttatgg gtgttttgtt tgtggctggg cctggccgac cctctctcca cggccttaac 481 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 541 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 601 tta