Construct: ORF ccsbBroad304_10605
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003461.1_s304c1, BRDN0000408861
- Derived from:
- ccsbBroadEn_10605
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM182A (284800)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10605
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284800 | FAM182A | family with sequence simila... | NR_026713.1 | 9% | 1_709del;1118G>A;1172_5111del | |
| 2 | human | 728882 | FAM182B | family with sequence simila... | NR_026714.2 | 7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 531
- ORF length:
- 462
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcaaggcatt gacagatgga tcccatcgat acccccactg caaaaccata 121 gtgcagtgaa tagaagacac ttctctgcac cccaaaagct ccatgctgat tgtgagaagg 181 aaatgctgga tggaggggtt cctggaacta ctgtgagggt ttcttttgat ttctcctgct 241 tagaaatggt gagagaactt tggatgtgga acgtggagga ggaggaacac gaagtgggga 301 tctccacttg gggtggtcag cactgcgggt gcccagcaaa gtcactgcct ggtccacatc 361 ccggaggggt ctctgcgcct cagtcggcat cgcagctgat ggtgaaactt ttggtgtggc 421 agaagagtgt ccacaaactt cggaagatcg ctgctcagag tgaggaatgg agttgtattt 481 tgaataaaat atccacgcat ccttttgtga gcaaggagca tgatggtact ttgccaactt 541 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 601 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta