Construct: ORF ccsbBroad304_10606
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006961.1_s304c1, BRDN0000404761
- Derived from:
- ccsbBroadEn_10606
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC285074 (285074)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10606
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64795 | RMND5A | required for meiotic nuclea... | NM_022780.4 | 24.8% | 24.8% | (many diffs) |
| 2 | human | 285074 | LOC285074 | anaphase promoting complex ... | NR_026846.1 | 13.1% | 1_353del;526G>C;648_2225del | |
| 3 | mouse | 68477 | Rmnd5a | required for meiotic nuclea... | NM_001355745.1 | 23.9% | 24.8% | (many diffs) |
| 4 | mouse | 68477 | Rmnd5a | required for meiotic nuclea... | NM_024288.2 | 23.9% | 24.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 360
- ORF length:
- 294
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tcagtgcgtg acggtggagc gcgagctgga gaaggtgctg cacaagttct 121 cgggctacgg gcagctgtgc gagcgcggcc tggaggagct catcgactac accggcggtc 181 tcaagcacga gatcctgcag agccacggcc aagatgctga attatcaggg acactttcac 241 ttgttttgac acagtgctgt aaaagaataa aggatactgt tcaaaaattg gcctccgacc 301 acaaagacat ccacagcagt gtttctcggg ttggaaaagc cattgataag gattcacttt 361 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 421 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 481 ggatta