Construct: ORF ccsbBroad304_10643

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016922.1_s304c1, BRDN0000407322
Derived from:
ccsbBroadEn_10643
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LOC440700 (440700)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10643
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 440700 LOC440700 carbonic anhydrase 14 pseud... NR_036683.1 12.2% 1_312del;322C>N;463_1216del
2 human 101928080 LOC101928080 uncharacterized LOC101928080 XR_930620.2 6.7% (many diffs)
3 human 101928080 LOC101928080 uncharacterized LOC101928080 XR_930619.2 6.5% (many diffs)
4 human 154214 RNF217 ring finger protein 217 XR_001743180.2 4.9% (many diffs)
5 human 107984660 LOC107984660 uncharacterized LOC107984660 XR_001750645.1 4.2% (many diffs)
6 human 23357 ANGEL1 angel homolog 1 NM_001370748.1 3.5% 3% (many diffs)
7 human 107984660 LOC107984660 uncharacterized LOC107984660 XR_001750646.1 3.4% (many diffs)
8 human 23357 ANGEL1 angel homolog 1 NM_001370746.1 3.2% 2.8% (many diffs)
9 human 107984660 LOC107984660 uncharacterized LOC107984660 XR_001750641.1 3.2% (many diffs)
10 human 105372107 LOC105372107 uncharacterized LOC105372107 XR_002958223.1 1.9% (many diffs)
11 human 105372107 LOC105372107 uncharacterized LOC105372107 XR_002959042.1 1.9% (many diffs)
12 human 6569 SLC34A1 solute carrier family 34 me... XR_941113.2 1.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
216
ORF length:
150
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg gtggntcaca cctgtaatcc cagcactttg ggaggcggag gtgggtggat
121tgcctgagct caggagttcc agaccagcct ggacaacatg gtggactttc cagaaaatat
181gtagctaccc agctacacct gcactggggt caggatgccc aactttcttg tacaaagtgg
241ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
301ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)