Construct: ORF ccsbBroad304_10660
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004973.1_s304c1, BRDN0000412068
- Derived from:
- ccsbBroadEn_10660
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC646938 (646938)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10660
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 646938 | LOC646938 | TBC1 domain family member 2... | NR_036495.1 | 52.5% | (many diffs) | |
| 2 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XM_017022022.1 | 10.5% | 8.7% | (many diffs) |
| 3 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XM_011521387.2 | 8.3% | 6.9% | (many diffs) |
| 4 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | NM_144572.1 | 8% | 6.6% | (many diffs) |
| 5 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XR_001751162.1 | 4.5% | (many diffs) | |
| 6 | human | 23102 | TBC1D2B | TBC1 domain family member 2B | XR_001751163.1 | 3.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 324
- ORF length:
- 255
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggacaggc aggaagtcct tcggggcatt tctgtttcat cacagctttt 121 cctggggctg gaagctgatc agtatctcct ttggggacct gaaccctttc cccgtacgcc 181 agatccggaa ccaacgcgcc taccacttgg agaaagtccg gctggagctg accgagctgg 241 aggccatccg tgaggacttc ctgcatgagc gggacaccag ccctgacaag ggtgagctgg 301 tcagtgacga ggaggaggat accttgccaa ctttcttgta caaagtggtt ggtaagccta 361 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 421 gcgttaagtc gacaatcaac ctctggatta