Construct: ORF ccsbBroad304_10819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008019.1_s304c1, BRDN0000401128
- Derived from:
- ccsbBroadEn_10819
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FCER1G (2207)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10819
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2207 | FCER1G | Fc fragment of IgE receptor Ig | NM_004106.2 | 98.8% | 98.8% | 141_142insGTA |
| 2 | mouse | 14127 | Fcer1g | Fc receptor, IgE, high affi... | NM_010185.4 | 87.3% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 327
- ORF length:
- 261
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat tccagcagtg gtcttgctct tactcctttt ggttgaacaa gcagcggccc 121 tgggagagcc tcagctctgc tatatcctgg atgccatcct gtttctgtat ggaattgtcc 181 tcaccctcct ctactgtcga ctgaaggtaa tccaagtgcg aaaggcagct ataaccagct 241 atgagaaatc agatggtgtt tacacgggcc tgagcaccag gaaccaggag acttacgaga 301 ctctgaagca tgagaaacca ccacagtacc caactttctt gtacaaagtg gttggtaagc 361 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 421 ggcgcgttaa gtcgacaatc aacctctgga tta