Construct: ORF ccsbBroad304_10849

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018216.1_s304c1, BRDN0000398802
Derived from:
ccsbBroadEn_10849
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GMFB (2764)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10849
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2764 GMFB glia maturation factor beta NM_004124.3 91.7% 91.5% 418delT;426_427ins37
2 mouse 63985 Gmfb glia maturation factor, beta NM_022023.2 83.5% 89.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
528
ORF length:
462
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag tgagtctttg gttgtttgtg atgttgccga agatttagtg gaaaagctga
121gaaagtttcg ttttcgcaaa gaaacgaaca acgctgctat tataatgaag attgacaagg
181ataaacgcct ggtggtactg gatgaggagc ttgagggcat ttcaccagat gaacttaaag
241atgaactacc tgaacgacaa cctcgcttca ttgtgtatag ttataaatat caacatgatg
301atggaagagt ttcatatcct ctgtgcttta ttttctccag tcctgttgga tgtaagcctg
361aacaacagat gatgtatgct ggaagtaaga ataagctagt ccagacagct gaactaacca
421aggtatttga aataagaaat accgaagacc taactgaaga atggttacgt gagaaacttg
481gatttttcac taatgtgaac ttctgtgttt ctaaagtatt tatgtattac ccaactttct
541tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
601aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)